Tianjin Institute for Food Safety Inspection Technology, Tianjin 300308, China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
Oxid Med Cell Longev. 2022 Jun 10;2022:9973419. doi: 10.1155/2022/9973419. eCollection 2022.
The present study investigated physicochemical properties and antioxidant activities and of purified compound polysaccharides (CPs-1) from Chinese herbal medicines, composed of lotus leaf, hawthorn, Fagopyrum tataricum, Lycium barbarum, Semen cassiae, and Poria cocos with the mass ratio of 2 : 4 : 2 : 1 : 1.5 : 1. The HPGPC profile and FT-IR spectra indicated that the average molecular weight of CPs-1 was 38.7 kDa and possessed the - and -D-pyranose, respectively. The methylation analysis and NMR spectrum demonstrated that CPs-1 had a →6)--D-Glcp-(1→6)--D-Glcp(1→ backbone. Furthermore, the antioxidant assays revealed that CPs-1 displayed high scavenging abilities for DPPH, hydroxyl, and reducing power, as well as ABTS and superoxide scavenging capacity. The antioxidant experiments revealed that CPs-1 could significantly enhance CAT, SOD, and GSH-Px activities and dramatically reduce MDA levels in liver and serum of high-fat mice. Therefore, CPs-1 could be potentially incorporated into pharmaceutical products or functional foods as a natural antioxidant.
本研究以荷叶、山楂、苦荞麦、枸杞、决明子、茯苓质量比为 2∶4∶2∶1.5∶1 的比例，提取、分离、纯化中草药复合多糖（CPs-1），研究其理化性质和抗氧化活性。HPGPC 图谱和 FT-IR 光谱表明，CPs-1 的平均分子量为 38.7 kDa，分别具有-β-D-吡喃糖和-α-D-吡喃糖。甲基化分析和 NMR 谱表明，CPs-1 具有→6）--D-Glcp-(1→6)--D-Glcp(1→的主链。此外，抗氧化测定结果表明，CPs-1 对 DPPH、羟基和还原能力具有较高的清除能力，以及 ABTS 和超氧阴离子清除能力。抗氧化实验表明，CPs-1 可显著提高高脂小鼠肝和血清中 CAT、SOD 和 GSH-Px 的活性，显著降低 MDA 水平。因此，CPs-1 可作为一种天然抗氧化剂，潜在地应用于医药产品或功能性食品中。
