用fura-2测量分离的神经分泌神经末梢中去极化诱导的Ca2+增加。
Depolarization-induced Ca2+ increase in isolated neurosecretory nerve terminals measured with fura-2.
作者信息
Brethes D, Dayanithi G, Letellier L, Nordmann J J
出版信息
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1439-43. doi: 10.1073/pnas.84.5.1439.
Abstract
The free Ca2+ concentration in isolated rat neurohypophysial nerve endings was measured using the Ca2+ indicator fura-2. Depolarization with high K, veratridine, or electrical stimulation induced an increase in intracellular Ca2+ concentration that was abolished by agents known to block voltage-sensitive Ca channels. Electrical stimulation of the isolated nerve endings with a pulse pattern similar to that recorded in vivo from the hypothalamic magnocellular neurons showed that the increase in intracellular Ca2+ concentration was not only a function of the applied frequency but also of the duration of the silent interburst intervals. The relationship between the cytoplasmic free Ca concentration and the release of neuropeptides is discussed.
摘要
利用钙离子指示剂fura-2测量了分离的大鼠神经垂体神经末梢中的游离钙离子浓度。用高钾、藜芦碱进行去极化或电刺激可诱导细胞内钙离子浓度升高，而这种升高可被已知能阻断电压敏感性钙通道的药物所消除。用类似于从下丘脑大细胞神经元体内记录到的脉冲模式对分离的神经末梢进行电刺激，结果表明细胞内钙离子浓度的升高不仅是施加频率的函数，也是沉默爆发间期持续时间的函数。本文讨论了细胞质游离钙浓度与神经肽释放之间的关系。
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