Medical Biochemistry Department, Medical Research Division, National Research Centre, Giza, Egypt.
Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Toxicol Mech Methods. 2022 May;32(4):268-279. doi: 10.1080/15376516.2021.1998275. Epub 2021 Nov 10.
Alpha-lipoic acid (α-LA) is characterized by its unpleasant odor, poor bioavailability and stability. Nanotechnology was applied to overcome this limitation. So we aimed in this study to formulate α-LA in two different forms of chitosan nanoparticles (CsNPs) and solid lipid nanoparticles (SLNPs) and characterize them in terms of physical properties and biological activities against aluminum chloride (AlCl)-induced neurotoxicity in rats. The study was processed on 50 rats divided into 5 groups as follow: control, neurotoxic, treated α-LA, treated α-lipoic acid-loaded chitosan nanoparticles (α-LA-CsNPs) and treated α-lipoic acid-loaded solid lipid nanoparticles (α-LA-SLNPs) groups. The result was depicted by transmission electron microscopy (TEM) revealed that α-LA-SLNPs had a regular spherical shape while α-LA-CsNPs showed an irregular spherical form. Dynamic light scattering (DLS) analysis showed that the average particle size for α-LA-SLNPs was about 71 nm and for α-LA-CsNPs was about 126 nm. After the experimental period, we observed that AlCl administration significantly increased oxidative stress, neuroinflammation and apoptosis and decreased brain fatty acid contentsand brain-derived neurotrophic factor,while α-LA, α-LA-CsNPs and α-LA-SLNPs were able to ameliorate these negative changes in the neurotoxic rats. However, the effect of the α-LA-loaded NPs was more prominent than that of pristine α-LA but the α-LA-SLNPs group was almost close to the control group. α-LA can attenuate neurotoxicity induced by AlCl3, attributed to its anti-inflammatory, antioxidant and anti-apoptotic activities in addition to the effectiveness of the encapsulation technique that can increase the efficiency and stability of α-LA. Moreover, α-LA-SLNPs are more efficient than α-LA-CsNPs.
α-硫辛酸（α-LA）具有不良气味、生物利用度差和稳定性差的特点。纳米技术被应用于克服这一限制。因此，我们旨在本研究中将 α-LA 制成两种不同形式的壳聚糖纳米粒子（CsNPs）和固体脂质纳米粒子（SLNPs），并从物理性质和对大鼠氯化铝（AlCl）诱导神经毒性的生物活性两方面对其进行表征。该研究在 50 只大鼠上进行，分为 5 组：对照组、神经毒性组、α-LA 治疗组、负载 α-LA 的壳聚糖纳米粒子（α-LA-CsNPs）治疗组和负载 α-LA 的固体脂质纳米粒子（α-LA-SLNPs）治疗组。透射电子显微镜（TEM）的结果显示，α-LA-SLNPs 具有规则的球形形状，而 α-LA-CsNPs 则呈现出不规则的球形形态。动态光散射（DLS）分析显示，α-LA-SLNPs 的平均粒径约为 71nm，α-LA-CsNPs 的平均粒径约为 126nm。在实验期间，我们观察到 AlCl 给药显著增加了氧化应激、神经炎症和细胞凋亡，降低了大脑脂肪酸含量和脑源性神经营养因子，而 α-LA、α-LA-CsNPs 和 α-LA-SLNPs 能够改善神经毒性大鼠的这些负面变化。然而，负载 NPs 的 α-LA 的效果比原 α-LA 更显著，但 α-LA-SLNPs 组几乎接近对照组。α-LA 可以减轻 AlCl3 诱导的神经毒性，这归因于其抗炎、抗氧化和抗细胞凋亡活性，以及封装技术的有效性，该技术可以提高 α-LA 的效率和稳定性。此外，α-LA-SLNPs 比 α-LA-CsNPs 更有效。
