Awadh Najma, Nyawale Helmut, Chibwe Elieza, Mujuni Fridolin, Ollomi Margareth, Hassan Karim, Mtebe Majigo, Matemba Lucas, Mshana Stephen E, Mirambo Mariam M
Department of Obstetrics and Gynecology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza 1464, Tanzania.
Department of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza 1464, Tanzania.
Trop Med Infect Dis. 2021 Oct 19;6(4):186. doi: 10.3390/tropicalmed6040186.
Viral infections have been associated with poor pregnancy outcomes. We investigated the magnitude of rubella virus (RV), dengue virus (DENV), Zika virus (ZIKV) and human cytomegalovirus (HCMV) among symptomatic pregnant women in rural and urban areas of Mwanza. A cross-sectional study was conducted between July 2017 and April 2018 in Mwanza. A rapid immunochromatographic test was done to detect ZIKV IgM and IgG as well as DENV IgM and IgG antibodies. A multiplex_RT-PCR was also done to detect the viral RNA genome. Enzyme immunoassays were done to detect RV and HCMV. Out of 171 participants, 1 (0.6%) was found to be seropositive for ZIKV_IgM antibodies, while 5 (2.9%) were ZIKV_IgG seropositive. DENV seropositivity was 9 (5.3%) and 3 (1.8%) for IgM and IgG, respectively, with all being PCR negative. Two participants (1.2%) were RV_IgM seropositive. 100% were HCMV_IgG seropositive and none was HCMV_IgM seropositive. Among 70 women with high HCMV_IgG titters, 10 (14.3%) had a low avidity index, indicating recent infections. Residing in rural areas ( = 0.044) and advanced age ( = 0.024) independently predicted ZIKV/DENV seropositivity. A substantial proportion of pregnant women had markers for viral infections. There is a need for introducing routine screening and monitoring pregnancy outcomes of positive cases to establish the relationship of these viruses and adverse pregnancy outcomes in endemic areas.
病毒感染与不良妊娠结局有关。我们调查了姆万扎城乡有症状孕妇中风疹病毒（RV）、登革病毒（DENV）、寨卡病毒（ZIKV）和人巨细胞病毒（HCMV）的感染情况。2017年7月至2018年4月在姆万扎进行了一项横断面研究。采用快速免疫层析试验检测寨卡病毒IgM和IgG以及登革病毒IgM和IgG抗体。还进行了多重逆转录聚合酶链反应以检测病毒RNA基因组。采用酶免疫测定法检测风疹病毒和人巨细胞病毒。在171名参与者中，发现1人（0.6%）寨卡病毒IgM抗体血清学阳性，5人（2.9%）寨卡病毒IgG血清学阳性。登革病毒IgM和IgG血清学阳性率分别为9人（5.3%）和3人（1.8%），所有PCR检测均为阴性。两名参与者（1.2%）风疹病毒IgM血清学阳性。100%的人巨细胞病毒IgG血清学阳性，无人巨细胞病毒IgM血清学阳性。在70名巨细胞病毒IgG滴度高的女性中，10人（14.3%）亲和力指数低，表明近期感染。居住在农村地区（P = 0.044）和高龄（P = 0.024）独立预测寨卡病毒/登革病毒血清学阳性。相当一部分孕妇有病毒感染标志物。有必要引入常规筛查并监测阳性病例的妊娠结局，以确定这些病毒与流行地区不良妊娠结局之间的关系。
