Mirambo Mariam Mwinjuma, Senyaeli Ndealilia, Mshana Stephen Eliatosha
Department of Microbiology and Immunology, Weill Bugando School of Medicine, P.O. Box 1464, Mwanza, Tanzania.
Pan Afr Med J. 2017 Oct 10;28:131. doi: 10.11604/pamj.2017.28.131.10480. eCollection 2017.
Human cytomegalovirus (HCMV) is one of the opportunistic infections associated with significant morbidity and mortality among HIV/AIDS patients especially before introduction of antiretroviral therapy (ART). Little is known regarding the humoral immune response against HCMV in relation to CD4 counts among HIV infected individuals. A total of 90 achieved sera from HIV infected patients attending Bugando Medical centre care and treatment centre (CTC) aged 18 years and above were retrieved and analyzed. Sociodemographic data were collected using structured data collection tool. Detection of specific HCMV antibodies was done using Indirect Enzyme Linked Immunosorbent Assay (ELISA). Data were analyzed by using STATA version 11. A total of 90 HIV infected patients were enrolled in the study whereby 36(40%) had immunological treatment failure. The mean age of the study participants was 39±12.3 years. The Prevalence of specific HCMV IgG antibodies was 84(93.3%, 95% CI: 88-98.5) while the prevalence of specific HCMV IgM antibodies was 2(2.3% 95% CI: 0.8-5.4). The median CD4 counts at 6 months and 12 months on HAART were significantly high in treatment success group. At 12 months of HAART as CD4 counts increases the HCMV IgG index value was also found to increase significantly, p=0.04. Significant proportion of HIV infected individuals was infected with HCMV. Higher median HCMV IgG titers were observed among patients with immunological treatment success. There is a need to investigate humoral immune responses in HIV infected individuals in relation to CD4 counts against various infectious diseases in developing countries where most of these infections are endemic.
人类巨细胞病毒（HCMV）是与艾滋病毒/艾滋病患者显著发病和死亡相关的机会性感染之一，尤其是在抗逆转录病毒疗法（ART）引入之前。关于艾滋病毒感染者中针对HCMV的体液免疫反应与CD4细胞计数的关系，人们知之甚少。从布甘多医疗中心护理和治疗中心（CTC）就诊的18岁及以上艾滋病毒感染患者中总共获取并分析了90份血清。使用结构化数据收集工具收集社会人口统计学数据。使用间接酶联免疫吸附测定（ELISA）检测特异性HCMV抗体。使用STATA 11版分析数据。共有90名艾滋病毒感染患者纳入该研究，其中36人（40%）有免疫治疗失败情况。研究参与者的平均年龄为39±12.3岁。特异性HCMV IgG抗体的患病率为84（93.3%，95%可信区间：88 - 98.5），而特异性HCMV IgM抗体的患病率为2（2.3%，95%可信区间：0.8 - 5.4）。在治疗成功组中，接受高效抗逆转录病毒治疗（HAART）6个月和12个月时的CD4细胞计数中位数显著较高。在HAART治疗12个月时，随着CD4细胞计数增加，HCMV IgG指数值也显著增加，p = 0.04。相当比例的艾滋病毒感染者感染了HCMV。在免疫治疗成功的患者中观察到更高的HCMV IgG滴度中位数。在大多数这些感染为地方病的发展中国家，有必要研究艾滋病毒感染者中针对各种传染病的体液免疫反应与CD4细胞计数的关系。
