Lambert Élie, Puwakdandawa Kavindu, Tao Yi Fei, Robert François
Institut de recherches cliniques de Montréal, Canada.
Département de Médecine, Faculté de Médecine, Université de Montréal, Canada.
FEBS J. 2023 Jan;290(2):286-309. doi: 10.1111/febs.16250. Epub 2021 Nov 11.
Mediator is a large modular protein assembly whose function as a coactivator of transcription is conserved in all eukaryotes. The Mediator complex can integrate and relay signals from gene-specific activators bound at enhancers to activate the general transcription machinery located at promoters. It has thus been described as a bridge between these elements during initiation of transcription. Here, we review recent studies on Mediator relating to its structure, gene specificity and general requirement, roles in chromatin architecture as well as novel concepts involving phase separation and transcriptional bursting. We revisit the mechanism of action of Mediator and ultimately put forward models for its mode of action in gene activation.
中介体是一种大型模块化蛋白质组装体，其作为转录共激活因子的功能在所有真核生物中都是保守的。中介体复合物可以整合并传递来自结合在增强子上的基因特异性激活因子的信号，以激活位于启动子处的通用转录机制。因此，它被描述为转录起始过程中这些元件之间的桥梁。在这里，我们综述了关于中介体的最新研究，这些研究涉及其结构、基因特异性和一般要求、在染色质结构中的作用以及涉及相分离和转录爆发的新概念。我们重新审视了中介体的作用机制，并最终提出了其在基因激活中的作用模式模型。
