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中介体复合物的功能和动力学：新的见解和新的前沿。

Function and dynamics of the Mediator complex: novel insights and new frontiers.

机构信息

Wadsworth Center, New York State Department of Health, Albany, NY, United States.

Department of Biomedical Sciences, University at Albany School of Public Health, Albany, NY, United States.

出版信息

Transcription. 2022 Feb-Jun;13(1-3):39-52. doi: 10.1080/21541264.2022.2085502. Epub 2022 Jun 16.

DOI:10.1080/21541264.2022.2085502
PMID:35708525
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9467533/
Abstract

The Mediator complex was discovered in the early 1990s as a biochemically fractionated factor from yeast extracts that was necessary for activator-stimulated transcriptional activation to be observed in transcription assays. The structure of this large, multi-protein complex is now understood in great detail, and novel genetic approaches have provided rich insights into its dynamics during transcriptional activation and the mechanism by which it facilitates activated transcription. Here I review recent findings and unanswered questions regarding Mediator dynamics, the roles of individual subunits, and differences between its function in yeast and metazoan cells.

摘要

中介复合物于 20 世纪 90 年代初在酵母提取物中被发现，是一种生化分离因子，在转录测定中，它对于激活剂刺激的转录激活是必需的。该大型多蛋白复合物的结构现在已经有了非常详细的了解，新的遗传方法为深入了解其在转录激活过程中的动力学以及它促进激活转录的机制提供了丰富的见解。在这里，我回顾了关于中介复合物动力学、各个亚基的作用以及其在酵母和后生动物细胞中的功能差异的最新发现和未解决的问题。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/77f3b79010bb/KTRN_A_2085502_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/8af8cd6cf007/KTRN_A_2085502_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/e713015fbea8/KTRN_A_2085502_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/f57a6e4f78ff/KTRN_A_2085502_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/13ab7ccd50a1/KTRN_A_2085502_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/77f3b79010bb/KTRN_A_2085502_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/8af8cd6cf007/KTRN_A_2085502_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/e713015fbea8/KTRN_A_2085502_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/f57a6e4f78ff/KTRN_A_2085502_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/13ab7ccd50a1/KTRN_A_2085502_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d064/9467533/77f3b79010bb/KTRN_A_2085502_F0005_OC.jpg

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本文引用的文献

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2
Mediator dynamics during heat shock in budding yeast.在芽殖酵母的热激过程中介质的动力学。
Genome Res. 2022 Jan;32(1):111-123. doi: 10.1101/gr.275750.121. Epub 2021 Nov 16.
3
From structure to molecular condensates: emerging mechanisms for Mediator function.从结构到分子凝聚物：中介体功能的新出现机制
利用功能无细胞提取物在单分子分辨率下解析核糖核蛋白复合物生物学。
Wiley Interdiscip Rev RNA. 2023 Sep-Oct;14(5):e1787. doi: 10.1002/wrna.1787. Epub 2023 Apr 12.
4
Transcription machinery of the minimalist: comparative genomic analysis provides insights into the (de)regulated transcription mechanism of microsporidia - fungal-relative parasites.极简生物的转录机制：比较基因组分析深入了解微孢子虫——真菌相关寄生虫的（去）调控转录机制。
Transcription. 2023 Nov;14(1-2):1-17. doi: 10.1080/21541264.2023.2174765. Epub 2023 Feb 9.
FEBS J. 2023 Jan;290(2):286-309. doi: 10.1111/febs.16250. Epub 2021 Nov 11.
4
Comparison of transcriptional initiation by RNA polymerase II across eukaryotic species.真核生物中 RNA 聚合酶 II 转录起始的比较。
Elife. 2021 Sep 13;10:e67964. doi: 10.7554/eLife.67964.
5
Connection of core and tail Mediator modules restrains transcription from TFIID-dependent promoters.核心和尾部 Mediator 模块的连接抑制了 TFIID 依赖性启动子的转录。
PLoS Genet. 2021 Aug 12;17(8):e1009529. doi: 10.1371/journal.pgen.1009529. eCollection 2021 Aug.
6
Spatiotemporal coordination of transcription preinitiation complex assembly in live cells.转录起始前复合物在活细胞中的时空协调组装。
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The Pol II preinitiation complex (PIC) influences Mediator binding but not promoter-enhancer looping.Pol II 起始前复合物（PIC）影响 Mediator 结合，但不影响启动子增强子环。
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