Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, Department of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, 130021, China.
Jilin Province of Jilin Combine Traditional Chinese and Western Medicine Hospital, Jilin, 132000, China.
Genes Genomics. 2022 Jan;44(1):97-108. doi: 10.1007/s13258-021-01173-1. Epub 2021 Oct 26.
Long non-coding RNAs (lncRNAs) are widely involved in gene transcription regulation and which act as epigenetic modifiers in many diseases.
To determine whether lncRNAs are involved in ischemic stroke (IS), we analyzed the expression profile of lncRNAs and mRNAs in IS.
RNA sequencing was performed on the blood of three pairs of IS patients and healthy controls. Differential expression analysis was used to identify differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs). Based on the co-expression relationships between lncRNA and mRNA, a series of bioinformatics analysis including GO and KEGG enrichment analysis and PPI analysis, were conducted to predict the function of lncRNA.
RNA sequencing produced a total of 5 DElncRNAs and 144 DEmRNAs. Influenza A pathway and Herpes simplex infection pathway were the most significant pathways. EP300 and NFKB1 were the most important target proteins, and Human leucocyte antigen (HLA) family were the key genes in IS.
Analysis of this study revealed that dysregulated lncRNAs in IS may lead to IS by affecting the immune and inflammation system.
长链非编码 RNA（lncRNA）广泛参与基因转录调控，并在许多疾病中作为表观遗传修饰物发挥作用。
为了确定 lncRNA 是否参与缺血性脑卒中（IS），我们分析了 IS 患者血液中的 lncRNA 和 mRNA 的表达谱。
对 3 对 IS 患者和健康对照者的血液进行 RNA 测序。使用差异表达分析来鉴定差异表达的 lncRNA（DElncRNA）和 mRNA（DEmRNA）。基于 lncRNA 和 mRNA 之间的共表达关系，进行了一系列生物信息学分析，包括 GO 和 KEGG 富集分析和 PPI 分析，以预测 lncRNA 的功能。
RNA 测序共产生了 5 个 DElncRNA 和 144 个 DEmRNA。流感 A 途径和单纯疱疹感染途径是最显著的途径。EP300 和 NFKB1 是最重要的靶蛋白，人类白细胞抗原（HLA）家族是 IS 中的关键基因。
本研究的分析表明，IS 中失调的 lncRNA 可能通过影响免疫和炎症系统导致 IS。
