Institute of Metabonomics & Medical NMR, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou325035, China.
J Proteome Res. 2021 Nov 5;20(11):5024-5035. doi: 10.1021/acs.jproteome.1c00519. Epub 2021 Oct 26.
Diabetic enteropathy (DE) is a diabetic complication and affects the quality of life for which there are limited therapies. In this study, / mice were administered with a basic fibroblast growth factor (bFGF) to explore its therapeutic effect on the intestine. H NMR-based metabolomics was applied to investigate the metabolic pattern. H&E and PAS staining were used to observe the morphological phenotypes related to intestinal barrier function. Tight junction proteins such as Zo-1 and Occluding were successively tested by immunofluorescence and real-time PCR. We found that bFGF treatment significantly restored intestinal barrier function. In addition, the administration of bFGF decreased the levels of inflammatory cytokines in the cecum. Metabolomic results show that bFGF remodeled metabolic phenotypes of the colon, cecum, and small intestine in / mice, including energy metabolism, short chain fatty acid metabolism, amino acid metabolism, and choline metabolism. Hence, this study indicates that the bFGF has a protective effect in diabetic bowel disease by restoring intestinal barrier function, reducing inflammatory infiltration, and remodeling metabolic function.
糖尿病性肠病（DE）是一种糖尿病并发症，影响生活质量，目前治疗方法有限。本研究中，给 / 小鼠施用碱性成纤维细胞生长因子（bFGF），以探讨其对肠道的治疗作用。采用基于核磁共振的代谢组学研究来探讨代谢模式。通过 H&E 和 PAS 染色观察与肠道屏障功能相关的形态表型。通过免疫荧光和实时 PCR 相继测试紧密连接蛋白如 Zo-1 和 Occluding。我们发现 bFGF 治疗可显著恢复肠道屏障功能。此外，bFGF 的给药可降低盲肠中炎性细胞因子的水平。代谢组学结果表明，bFGF 重塑了 / 小鼠结肠、盲肠和小肠的代谢表型，包括能量代谢、短链脂肪酸代谢、氨基酸代谢和胆碱代谢。因此，本研究表明，bFGF 通过恢复肠道屏障功能、减少炎症浸润和重塑代谢功能对糖尿病性肠病具有保护作用。
