Division of Sensory Organs and Communication, Department of Biomedical and Clinical Sciences, Linköping University, Region Östergötland.
Division of ENT Diseases, Department of Clinical Sciences, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden.
Otol Neurotol. 2022 Feb 1;43(2):e198-e204. doi: 10.1097/MAO.0000000000003395.
To find out if nitric oxide (NO) can be locally produced in the middle ear and if chronic otitis media (COM) and acquired cholesteatoma affect the production.
Case-control study.
Two tertiary-referral hospitals.
Gaseous NO from 11 patients with unilateral perforations or grommet openings was measured with chemiluminescence. Middle ear mucosa from 48 patients with COM and 26 patients with cholesteatoma was investigated.
Detection of NO. Expression of nitric oxide synthase (NOS) mRNA, in mucosa from COM, cholesteatoma and healthy controls, quantified using polymerase chain reaction.
The gaseous NO from ears with a unilateral tympanic membrane perforation or a grommet was higher (9 ± 3 ppb, n = 11) than among the controls (4 ± 1 ppb, n = 11, p = 0.04). Lower levels of eNOS (2.64 ± 0.86 mol/100,000 mol ACTB) were detected in the pooled samples from the COM group (n = 48), compared with the control group (140.48 ± 92 mol/100,000 mol ACTB, n = 45, p = 0.010). In the cholesteatoma group (n = 26), a lower expression of nNOS (5.78 × 10-6 ± 1.13 × 10-6 ΔCt) was found in comparison with the controls (1.23 × 10-4 ± 3.18 × 10-5 ΔCt, n = 15, p = 0.011).
NO is likely a natural and permanent part of the gas mixture in the human middle ear. The presence of NOS enzymes in the middle ear mucosa indicates an ongoing NO production and the reduction of NOS in ears with cholesteatoma, and pooled samples from ears with COM, suggest a role for locally produced NO in middle ear disease.
探讨一氧化氮（NO）能否在中耳局部产生，以及慢性中耳炎（COM）和获得性胆脂瘤是否影响其产生。
病例对照研究。
两家三级转诊医院。
用化学发光法测量 11 例单侧鼓膜穿孔或鼓膜置管患者的气态 NO。对 48 例 COM 患者和 26 例胆脂瘤患者的中耳黏膜进行了研究。
NO 的检测。采用聚合酶链反应定量检测 COM、胆脂瘤和健康对照组黏膜中一氧化氮合酶（NOS）mRNA 的表达。
单侧鼓膜穿孔或鼓膜置管患者的气态 NO 高于对照组（9±3 ppb，n=11）（4±1 ppb，n=11，p=0.04）。与对照组（n=45，140.48±92 mol/100000 mol ACTB）相比，COM 组（n=48）的 eNOS 水平（2.64±0.86 mol/100000 mol ACTB）较低（p=0.010）。与对照组（n=15，1.23×10-4±3.18×10-5 ΔCt）相比，胆脂瘤组（n=26）的 nNOS 表达水平较低（5.78×10-6±1.13×10-6 ΔCt，p=0.011）。
NO 可能是人类中耳气体混合物中天然且永久性的组成部分。中耳黏膜中 NOS 酶的存在表明持续产生 NO，胆脂瘤中耳中 NOS 的减少，以及 COM 患者中耳黏膜样本的减少，表明局部产生的 NO 可能在中耳疾病中起作用。
