Peng Dazhao, Wei Cheng, Zhang Xiaoyang, Li Shenghui, Liang Hao, Zheng Xingyu, Jiang Shulong, Han Lei
Tianjin Neurological Institute, Key Laboratory of Post-Neuroinjury Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin Medical University General Hospital, 154 Anshan Road, Heping District, Tianjin, 300052, China.
Department of Gynecology and Obstetrics, Tianjin Medical University General Hospital, Tianjin, 300052, China.
Cancer Cell Int. 2021 Oct 26;21(1):566. doi: 10.1186/s12935-021-02266-3.
The function of collagen triple helix repeat containing 1 (CTHRC1) as an oncogene has been reported in a growing number of publications. Bioinformatics methods represent a beneficial approach to examine the mechanism and function of the CTHRC1 gene in the disease process of cancers from a pan-cancer perspective.
In this study, using the online databases UCSC, NCBI, HPA, TIMER2, Oncomine, GEPIA, UALCAN, cBioPortal, COSMIC, MEXPRESS, STRING, CCLE, LinkedOmics, GTEx, TCGA, CGGA, and SangerBox, we focused on the relationship between CTHRC1 and tumorigenesis, progression, methylation, immunity, and prognosis. qPCR was used to detect CTHRC1 expression in glioma tissues and cell lines.
The pan-cancer analysis showed that CTHRC1 was overexpressed in most tumors, and a significant correlation was observed between CTHRC1 expression and the prognosis of patients with cancer. CTHRC1 genetic alterations occur in diverse tumors and are associated with tumor progression. Levels of CTHRC1 promoter methylation were decreased in most cancer tissues compared with normal tissues. In addition, CTHRC1 coordinated the activity of ICP genes through diverse signal transduction pathways, was also associated with immune cell infiltration and the tumor microenvironment, and potentially represented a promising immunotherapy target. We identified CTHRC1-related genes across cancers using the GEPIA2 tool. The single-gene GO analysis of CTHRC1 across cancers showed that it was involved in some signaling pathways and biological processes, such as the Wnt signaling pathway, cell migration, and positive regulation of protein binding. The expression and function of CTHRC1 were also further verified in glioma tissues and cell lines.
CTHRC1 is overexpressed in various cancer types and functions as an important oncogene that may promote tumorigenesis and development through different mechanisms. CTHRC1 may represent an important therapeutic target for human cancers.
越来越多的出版物报道了含胶原三螺旋重复序列1（CTHRC1）作为一种癌基因的功能。生物信息学方法是一种有益的途径，可从泛癌角度研究CTHRC1基因在癌症疾病过程中的机制和功能。
在本研究中，我们利用在线数据库UCSC、NCBI、HPA、TIMER2、Oncomine、GEPIA、UALCAN、cBioPortal、COSMIC、MEXPRESS、STRING、CCLE、LinkedOmics、GTEx、TCGA、CGGA和SangerBox，重点研究了CTHRC1与肿瘤发生、进展、甲基化、免疫和预后之间的关系。采用qPCR检测CTHRC1在胶质瘤组织和细胞系中的表达。
泛癌分析显示CTHRC1在大多数肿瘤中高表达，且CTHRC1表达与癌症患者预后显著相关。CTHRC1基因改变发生在多种肿瘤中，并与肿瘤进展相关。与正常组织相比，大多数癌组织中CTHRC1启动子甲基化水平降低。此外，CTHRC1通过多种信号转导途径协调ICP基因的活性，还与免疫细胞浸润和肿瘤微环境相关，并且可能是一个有前景的免疫治疗靶点。我们使用GEPIA2工具鉴定了跨癌症的CTHRC1相关基因。跨癌症的CTHRC1单基因GO分析表明，它参与了一些信号通路和生物学过程，如Wnt信号通路、细胞迁移和蛋白质结合的正调控。CTHRC1的表达和功能也在胶质瘤组织和细胞系中得到了进一步验证。
CTHRC1在多种癌症类型中高表达，作为一个重要的癌基因，可能通过不同机制促进肿瘤发生和发展。CTHRC1可能是人类癌症的一个重要治疗靶点。
