He Andong, Wang Jingyun, Yang Xiaofeng, Liu Jia, Yang Xuesong, Wang Guang, Li Ruiman
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
International Joint Laboratory for Embryonic Development & Prenatal Medicine, Division of Histology and Embryology, Medical College, Jinan University, Guangzhou, China.
Proteomics Clin Appl. 2022 Mar;16(2):e2100053. doi: 10.1002/prca.202100053. Epub 2021 Nov 10.
Preeclampsia (PE) is a severe disease that endangers the safety of mothers and fetuses worldwide. In the absence of specific treatments, more studies on novel predictive and diagnostic biomarkers for PE are required.
Data-independent acquisition proteomics, with five biological replicates, was used to investigate the protein expression profiles of placental tissues from patients with PE and normal pregnant women.
In total, 52 differentially expressed proteins (DEPs) were identified, 34 of them were upregulated and 18 downregulated. Bioinformatics analyses revealed that PE was associated with multiple GO terms and KEGG pathways. Arginase-1 (ARG1), ferritin light chain (FTL), and RNA cytidine acetyltransferase (NAT10) were identified as hub proteins, which were further validated in placental tissues and maternal plasma by western blot and ELISA.
FTL expression was significantly lower in the placental tissues and early and late pregnancy plasma of patients with PE compared to that in normal pregnant women. This study is the first to propose that FTL may be a potential predictive and diagnostic biomarker for PE; it provides a proteomics insight for understanding the pathological mechanism of this disease.
子痫前期（PE）是一种严重疾病，在全球范围内危及母婴安全。由于缺乏特异性治疗方法，需要对PE的新型预测和诊断生物标志物进行更多研究。
采用具有五个生物学重复的数据非依赖采集蛋白质组学方法，研究PE患者和正常孕妇胎盘组织的蛋白质表达谱。
共鉴定出52种差异表达蛋白（DEP），其中34种上调，18种下调。生物信息学分析表明，PE与多个基因本体（GO）术语和京都基因与基因组百科全书（KEGG）通路相关。精氨酸酶-1（ARG1）、铁蛋白轻链（FTL）和RNA胞嘧啶乙酰转移酶（NAT10）被鉴定为枢纽蛋白，并通过蛋白质免疫印迹法和酶联免疫吸附测定法在胎盘组织和母体血浆中进一步验证。
与正常孕妇相比，PE患者胎盘组织以及妊娠早期和晚期血浆中的FTL表达显著降低。本研究首次提出FTL可能是PE的潜在预测和诊断生物标志物；它为理解该疾病的病理机制提供了蛋白质组学见解。
