Laboratoire de Chimie Physique Moléculaire, École Polytechnique Fédérale de Lausanne, EPFL SB ISIC LCPM, Station 6, CH-1015 Lausanne, Switzerland.
Department of Physics, University of Gothenburg, 412 96 Gothenburg, Sweden.
Anal Chem. 2021 Nov 9;93(44):14730-14736. doi: 10.1021/acs.analchem.1c03190. Epub 2021 Oct 27.
The analysis of glycans presents a significant challenge that arises from their isomeric heterogeneity. While high-resolution ion mobility spectrometry (IMS) has shown the ability to resolve subtly different glycan isomers, their unambiguous assignment remains difficult. Here, we demonstrate an infrared (IR) spectroscopic approach for identifying isomers in a glycan mixture. To display the feasibility of this approach, we have constructed a small database of cryogenic spectra of five lacto--fucopentaose (LNFP) and six disaccharide isomers and demonstrated that in the cases where they cannot be separated by IMS, we can use a cryogenic IR spectrum to identify the isomeric components of a mixture.
糖链分析存在一个重大挑战，源于其异构多样性。尽管高分辨率离子淌度谱（IMS）已显示出分辨略有不同糖链异构体的能力，但对其进行明确归属仍然困难。在此，我们展示了一种用于鉴定糖混合物中异构体的红外（IR）光谱方法。为了展示该方法的可行性，我们构建了一个包含五个乳果糖五糖（LNFP）和六个二糖异构体的低温光谱小型数据库，并证明在无法通过 IMS 分离的情况下，我们可以使用低温 IR 光谱来鉴定混合物的异构成分。
