Laboratoire de Chimie Physique Moléculaire , École Polytechnique Fédérale de Lausanne , EPFL SB ISIC LCPM, Station 6 , CH-1015 Lausanne , Switzerland.
Anal Chem. 2019 Apr 2;91(7):4876-4882. doi: 10.1021/acs.analchem.9b00659. Epub 2019 Mar 14.
The isomeric complexity of glycans make their analysis by traditional techniques particularly challenging. While the recent combination of ion mobility spectrometry (IMS) with cryogenic IR spectroscopy has demonstrated promise as a new technique for glycan analysis, this approach has been limited by the modest resolution of the ion mobility stage. In this work we report results from a newly developed instrument that combines ultrahigh-resolution IMS with cryogenic IR spectroscopy for glycan analysis. This apparatus makes use of the recent development in traveling-wave IMS called structures for lossless ion manipulation. The IMS stage allows the selection of glycan isomers that differ in collisional cross section by as little as 0.2% before injecting them into a cryogenic ion trap for IR spectral analysis. We compare our results to those using drift-tube IMS and highlight the advantages of the substantial increase in resolution. Application of this approach to glycan mixtures demonstrates our ability to isolate individual components, measure a cryogenic IR spectrum, and identify them using a spectroscopic database.
聚糖的异构复杂性使得传统技术对其分析特别具有挑战性。尽管最近将离子淌度谱（IMS）与低温红外光谱相结合已被证明是一种新的聚糖分析技术，但这种方法受到离子淌度阶段分辨率适中的限制。在这项工作中，我们报告了一种新开发的仪器的结果，该仪器将超高分辨率 IMS 与低温红外光谱相结合用于聚糖分析。该仪器利用了称为无损离子操纵结构的最新行波 IMS 发展。IMS 阶段允许选择碰撞截面相差仅 0.2%的聚糖异构体，然后将它们注入低温离子阱进行红外光谱分析。我们将我们的结果与使用漂移管 IMS 的结果进行比较，并强调分辨率大幅提高的优势。该方法在聚糖混合物中的应用证明了我们能够分离单个成分、测量低温红外光谱并使用光谱数据库对其进行识别的能力。
