School of Life Science, University of Applied Sciences and Arts Northwestern Switzerland, Hofackerstrasee 30, Muttenz CH-4132, Switzerland.
Biopox, Viale Maria Bakunin, 12 - CAP 80125 Naples, Italy.
Chem Commun (Camb). 2021 Nov 11;57(90):11960-11963. doi: 10.1039/d1cc04916j.
We report a method of glycosylated enzymes' surface immobilisation and stabilisation. The enzyme is immobilised at the surface of silica nanoparticles through the reversible covalent binding of vicinal diols of the enzyme glycans with a surface-attached boronate derivative. A soft organosilica layer of controlled thickness is grown at the silica surface, entrapping the enzyme and thus avoiding enzyme leaching. We demonstrate that this approach results not only in high and durable activity retention but also enzyme stabilisation.
我们报告了一种糖基化酶的表面固定化和稳定化方法。该酶通过酶糖的邻二醇与表面附着的硼酸酯衍生物的可逆共价键合固定在二氧化硅纳米粒子的表面。在二氧化硅表面生长一层可控厚度的软有机硅层，将酶包裹起来，从而避免酶的浸出。我们证明，这种方法不仅能保持较高且持久的酶活，还能稳定酶。
