Department of Gene Function and Phenomics, National Institute of Genetics, Mishima, Shizuoka, Japan.
Department of Genetics, School of Life Science, The Graduate University for Advanced Studies, SOKENDAI, Mishima, Shizuoka, Japan.
Methods Mol Biol. 2022;2382:225-232. doi: 10.1007/978-1-0716-1744-1_13.
Phragmoplasts are plant-specific microtubule structures that form cell plates at the cell division plane. During late anaphase, phragmoplasts emerge between daughter nuclei as the derivative of spindle microtubules, and centrifugally expand toward the cell cortex to build cell plates during telophase. Phragmoplasts are composed of short antiparallel microtubules decorated with various microtubule-associated proteins. Mutants of these microtubule-associated proteins exhibit defects in phragmoplast morphology. Quantification of phragmoplast morphology is indispensable for assessing the phenotypes of these mutants. Here, we describe a method to quantify the width of phragmoplasts.
成膜体是植物特有的微管结构，在细胞分裂平面形成细胞板。在后期末期，成膜体作为纺锤体微管的衍生物出现在子核之间，并在末期向细胞皮层中心辐射状扩展以构建细胞板。成膜体由短的平行微管组成，这些微管被各种微管相关蛋白所修饰。这些微管相关蛋白的突变体表现出成膜体形态的缺陷。成膜体形态的定量分析对于评估这些突变体的表型是必不可少的。在这里，我们描述了一种定量分析成膜体宽度的方法。
