LAQV-REQUINTE and Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Princess Margaret Cancer Center, University Health Network, Toronto, ON, Canada.
J Mater Chem B. 2021 Nov 24;9(45):9285-9294. doi: 10.1039/d1tb01360b.
Raman spectroscopy coupled with confocal microscopy offers an alternative bioimaging technique overcoming limitations associated with sensitivity, tissue penetration and image resolution. Allied to the surface-enhanced Raman scattering (SERS) properties of gold nanoparticles (AuNP), we designed SERS nanoprobes with enhanced properties and straightforward application as bio-labelling agents for gliomas. The ensuing nanoprobes coated with simple sugar units (galactose or glucose) allowed assessing information about their intracellular localization (vesicular structures), with impressive sensitivity towards complex environments and proved the ability to overcome biological auto-fluorescence and high penetration in tissues. We validate the use of sugars as an all-in-one vector (Raman reporter, conferring high stability, biocompatibility and affinity to glioma cells) as imaging agents using an impressive technique.
拉曼光谱结合共焦显微镜提供了一种替代的生物成像技术，可以克服与灵敏度、组织穿透和图像分辨率相关的限制。结合金纳米粒子（AuNP）的表面增强拉曼散射（SERS）特性，我们设计了具有增强性能的 SERS 纳米探针，并将其简单地用作神经胶质瘤的生物标记试剂。随后，用简单的糖单位（半乳糖或葡萄糖）涂覆的纳米探针可以评估有关其细胞内定位（囊泡结构）的信息，对复杂环境具有令人印象深刻的敏感性，并证明了克服生物自发荧光和在组织中高穿透性的能力。我们使用令人印象深刻的技术验证了使用糖作为一体式载体（拉曼报告器，赋予高稳定性、生物相容性和对神经胶质瘤细胞的亲和力）作为成像剂的用途。
