Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry & Department of Chemistry, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China.
School of Chemistry and Chemical Engineering, Jiangsu Normal University, Xuzhou, Jiangsu 221116, PR China.
Biosens Bioelectron. 2016 Nov 15;85:324-330. doi: 10.1016/j.bios.2016.04.094. Epub 2016 Apr 30.
Nitric Oxide (NO) is a significant gaseous signalling molecule in various pathological and physiological pathways, whereas many of its functions are still ambiguous in part because of the shortage of powerful detection approaches. Herein, we present a type of reaction-based surface-enhanced Raman scattering (SERS) nanoprobes, o-phenylenediamine-modified gold nanoparticles (AuNPs/OPD), to detect the level of the endogenous NO in living cells. The detection is achieved through the SERS variation of AuNPs/OPD caused by the reaction between NO and OPD on the surface of AuNPs. The proposed SERS nanoprobes have a good stability and a rapid response to NO within 30s Moreover, as a result of the reaction specificity coupled with SERS fingerprinting, AuNPs/OPD nanoprobes demonstrate high selectivity towards NO over other biologically relevant species with a sensitivity at 10(-7)M level. Thereby, this SERS strategy can be used for monitoring NO that is endogenously produced in living macrophages, indicating immense potential in studying NO-involved pathophysiological processes in biological systems.
一氧化氮（NO）是各种病理和生理途径中的一种重要气态信号分子，但其许多功能仍然不明确，部分原因是缺乏强大的检测方法。本文中，我们提出了一种基于反应的表面增强拉曼散射（SERS）纳米探针，即邻苯二胺修饰的金纳米粒子（AuNPs/OPD），用于检测活细胞内内源性 NO 的水平。该检测是通过 NO 与 AuNPs 表面上的 OPD 之间的反应导致 AuNPs/OPD 的 SERS 变化来实现的。所提出的 SERS 纳米探针具有良好的稳定性和对 NO 的快速响应，在 30s 内即可完成。此外，由于反应的特异性与 SERS 指纹图谱相结合，AuNPs/OPD 纳米探针对 NO 具有很高的选择性，对其他具有生物学意义的物质的灵敏度可达 10(-7)M 级。因此，这种 SERS 策略可用于监测活巨噬细胞中内源性产生的 NO，这表明其在研究生物系统中涉及 NO 的病理生理过程方面具有巨大的潜力。
