Ontario Cancer Institute, Campbell Family Cancer Research Institute, University of Toronto, Toronto, Ontario, Canada.
Bioconjug Chem. 2012 Sep 19;23(9):1726-30. doi: 10.1021/bc300214z. Epub 2012 Aug 16.
A porphyrin-phospholipid conjugate with quenched fluorescence was utilized to serve as both the Raman dye and a stabilizing, biocompatible surface coating agent on gold nanoparticles. Through simple synthesis and validation with spectroscopy and confocal microscopy, we show that this porphyrin-lipid stabilized AuNP is a novel SERS probe capable of cellular imaging. To date, this is the first use of porphyrin as a Raman reporter molecule for SERS based imaging.
利用一种荧光猝灭的卟啉 - 磷脂缀合物作为金纳米粒子的拉曼染料和稳定的、生物相容性的表面涂层试剂。通过简单的合成和光谱学及共聚焦显微镜验证，我们表明这种卟啉脂质稳定的 AuNP 是一种新型的 SERS 探针，能够进行细胞成像。迄今为止，这是首次将卟啉用作基于 SERS 的成像的拉曼报告分子。
