Department of General Surgery, İstanbul University-Cerrahpaşa, Cerrahpaşa Faculty of Medicine, İstanbul-Turkey.
Department of General Surgery, Kartal Dr. Lütfi Kırdar City Hospital, İstanbul-Turkey.
Ulus Travma Acil Cerrahi Derg. 2021 Nov;27(6):613-618. doi: 10.14744/tjtes.2020.00268.
Inadequate intestinal perfusion resulting from hemorrhagic shock negatively affects wound healing. In this experimental study, we aimed to evaluate the effects of resuscitation with hypertonic saline on colonic anastomosis in rats with controlled hemorrhagic shock.
A total of 24 male Wistar albino rats weighing between 200 and 250 g were used in this study. The rats were divided into four groups as: Control, hypotonic, isotonic, and hypertonic. Median laparotomy, colon resection, and colocolonic anastomosis were performed to the rats in the control group. After creating controlled hemorrhagic shock to rats in other three groups, resuscitation was achieved with hypotonic, isotonic, and hypertonic saline. After resuscitation, median laparotomy, colon resection, and colocolonic anastomosis were performed on rats in these three groups. On the 5th post-operative day, a median laparotomy was applied to the rats in all groups and anastomosis lines were evaluated. Anastomotic bursting pressure, tissue hydroxyproline level, and tissue fibrosis degree were compared between the groups.
There was no statistically significant difference between the groups in terms of anastomotic bursting pressure, tissue hydroxyproline level, and tissue fibrosis degree (respectively; p=0.320, p=0.537, p>0.05).
In rats with controlled hemorrhagic shock, resuscitation with hypertonic saline does not differ significantly from isotonic or hypotonic saline in terms of healing of colonic anastomosis.
出血性休克导致的肠道灌注不足会对伤口愈合产生负面影响。在这项实验研究中，我们旨在评估高渗盐水复苏对控制出血性休克大鼠结肠吻合口的影响。
本研究共使用了 24 只雄性 Wistar 白化大鼠，体重在 200 至 250 克之间。将大鼠分为四组：对照组、低渗组、等渗组和高渗组。对照组大鼠行中腹部切开术、结肠切除和结肠直肠吻合术。在其他三组大鼠中建立控制出血性休克后，用低渗、等渗和高渗盐水进行复苏。复苏后，三组大鼠均行中腹部切开术、结肠切除和结肠直肠吻合术。在第 5 天术后，对所有组的大鼠进行中腹部切开术，并评估吻合线。比较各组之间的吻合口破裂压、组织羟脯氨酸水平和组织纤维化程度。
在吻合口破裂压、组织羟脯氨酸水平和组织纤维化程度方面，各组之间无统计学差异（分别为；p=0.320、p=0.537、p>0.05）。
在控制出血性休克的大鼠中，高渗盐水复苏在结肠吻合口愈合方面与等渗或低渗盐水无显著差异。
