实验性胰腺炎大鼠中α-生育酚的作用及其剂量敏感性对胰腺炎形成的影响。

Effect of alpha-tocopherol and dose sensitivity on pancreatitis formation in rats with experimental pancreatitis.

机构信息

Department of General Surgery, Mersin University Faculty of Medicine, Mersin-Turkey.

Department of General Surgery, Van Provincial Health Directorate, Başkale State Hospital, Van-Turkey.

出版信息

Ulus Travma Acil Cerrahi Derg. 2021 Nov;27(6):605-612. doi: 10.14744/tjtes.2020.89054.

DOI:10.14744/tjtes.2020.89054

PMID:34710231

Abstract

BACKGROUND

Acute pancreatitis is an inflammatory disease accompanied by pancreatic inflammation characterized by acinar cell damage and leukocyte infiltration in the tissue. At present, mortality and morbidity rates are high despite the current treatment of pancreatitis; therefore, new studies and treatment studies are needed. In this study, the effects of alpha-tocopherol on different doses of L-arginine-induced experimental acute pancreatitis model were investigated.

METHODS

Thirty adult male Sprague-Dawley albino rats were randomly divided into four groups; control (sham) group (n=6), acute pancreatitis group (n=8), low-dose alpha-tocopherol (200 mg/kg once intraperitoneal [IP]) group (n=8), and high dose alpha-tocopherol (400 mg/kg once ip) group (n=8). Experimental acute pancreatitis model was created by a single IP dose of 5 g/kg of L-arginine. Alpha-tocopherol was administered in a single dose intraperitoneally, 30 min before the creation of the experimental model of acute pancreatitis induced by L-arginine induction in Groups 3 and 4. Tissue and blood samples were taken under anesthesia 72 h after L-arginine injection; then the rats were sacrificed by decapitation. Serum amylase, lipase, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-alpha, and C-reactive protein (CRP) levels were examined. Pancreatic tissue samples were examined under a light microscope for histopathological examination.

RESULTS

When the acute pancreatitis group (Group 2) was compared to the control group (Group 1), serum amylase, lipase, IL-1β, IL-6, TNF-alpha, and CRP levels were all significantly increased (p<0.05 for all). Histopathological examination showed significant difference in edema (p<0.001) and inflammation (p=0.007) scores. When the low (Group 3) and high (Group 4) dose alpha-tocopherol groups were compared to Group 2, amylase, lipase, IL-1β, IL-6, TNF-alpha, and CRP parameters were statistically significantly lower (p<0.05 for all). In the histopathological comparison of Groups 2, 3, and 4, edema and inflammation scores were decreased in Groups 3 and 4 compared to Group 2. Comparing Group 4 to Group 3, lipase (p<0.01), IL-6 (p=0.038), and TNF-alpha (p=0.002) levels were significantly decreased; no significant difference was observed in the histopathological evaluation.

CONCLUSION

Alpha-tocopherol was found to reduce inflammation and pancreatic damage in acute pancreatitis and was more effective in high doses.

摘要

背景

急性胰腺炎是一种炎症性疾病，伴有胰腺炎症，其特征是腺泡细胞损伤和组织中的白细胞浸润。目前，尽管目前对胰腺炎进行了治疗，但死亡率和发病率仍很高；因此，需要新的研究和治疗研究。在这项研究中，研究了α-生育酚对不同剂量 L-精氨酸诱导的实验性急性胰腺炎模型的影响。

方法

将 30 只成年雄性 Sprague-Dawley 白化大鼠随机分为四组；对照组（假手术）组（n=6），急性胰腺炎组（n=8），低剂量α-生育酚（200mg/kg 一次腹腔内[IP]）组（n=8）和高剂量α-生育酚（400mg/kg 一次 ip）组（n=8）。通过单次腹腔内注射 5g/kg L-精氨酸建立实验性急性胰腺炎模型。在第 3 组和第 4 组中，在诱导 L-精氨酸诱导的急性胰腺炎模型前 30 分钟，单次腹腔内给予α-生育酚。在注射 L-精氨酸后 72 小时，在麻醉下采集组织和血液样本；然后通过断头处死大鼠。检测血清淀粉酶、脂肪酶、白细胞介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α 和 C 反应蛋白（CRP）水平。在光镜下检查胰腺组织样本以进行组织病理学检查。

结果

与对照组（第 1 组）相比，急性胰腺炎组（第 2 组）的血清淀粉酶、脂肪酶、IL-1β、IL-6、TNF-α 和 CRP 水平均显著升高（所有 p<0.05）。组织病理学检查显示水肿（p<0.001）和炎症（p=0.007）评分有显著差异。与第 2 组相比，低（第 3 组）和高（第 4 组）剂量α-生育酚组的淀粉酶、脂肪酶、IL-1β、IL-6、TNF-α 和 CRP 参数均统计学显著降低（所有 p<0.05）。在第 2、3 和 4 组的组织病理学比较中，与第 2 组相比，第 3 组和第 4 组的水肿和炎症评分降低。与第 3 组相比，第 4 组的脂肪酶（p<0.01）、IL-6（p=0.038）和 TNF-α（p=0.002）水平显著降低；组织病理学评价无显著差异。