Özgül Halit, Tatar Cihad, Özer Bahri, Aydın Hüsnü, Sarı Soner, Özer Songül Peltek
Department of General Surgery, Haseki Training and Research Hospital, İstanbul-Turkey.
Ulus Travma Acil Cerrahi Derg. 2019 Jan;25(1):1-6. doi: 10.5505/tjtes.2018.30413.
Acute pancreatitis is a disease with high morbidity and mortality, despite all the advances in technology. The overall mortality rate of acute pancreatitis is 10%, whereas the mortality rate in infected necrotizing pancreatitis is approximately 35%. In this study, we aimed to establish acute pancreatitis in rats in order to try out the alpha-tocopherol treatment protocol and to reveal the results biochemically and histopathologically.
Twenty-four male male Sprague-Dawley rats weighing between 300 and 350 g were used in the study. In Group 1, 80 µg/kg of normal saline was subcutaneously injected into eight rats; in Group 2, 80 µg/kg of cerulein was subcutaneously injected into eight rats; and in Group 3, 80 µg/kg of cerulein was subcutaneously injected into eight rats. In addition, 30 mg/kg of alpha-tocopherol was intraperitoneally injected into eight rats.
The mean Schoenberg score, serum amylase, and lipase and Neutrophil Gelatinase-Associated Lipocalin (NGAL) levels were statistically significantly higher in Group 2 than in Group 1. The mean Schoenberg score and serum amylase and lipase levels were statistically significantly lower in Group 3 than in Group 2.
In this experimental study rat model of cerulein-induced acute pancreatitis, 30 mg/kg of alpha-tocopherol was injected intraperitoneally to examine its effect on pancreatitis. The improvement was observed in the histopathological examination of pancreatic tissues. We think that alpha-tocopherol may have a therapeutic effect on pancreatic tissue.
尽管技术有了诸多进步，但急性胰腺炎仍是一种发病率和死亡率都很高的疾病。急性胰腺炎的总体死亡率为10%，而感染性坏死性胰腺炎的死亡率约为35%。在本研究中，我们旨在建立大鼠急性胰腺炎模型，以试验α-生育酚治疗方案，并从生化和组织病理学方面揭示结果。
本研究使用了24只体重在300至350克之间的雄性Sprague-Dawley大鼠。第1组，8只大鼠皮下注射80微克/千克的生理盐水；第2组，8只大鼠皮下注射80微克/千克的雨蛙素；第3组，8只大鼠皮下注射80微克/千克的雨蛙素。此外，8只大鼠腹腔注射30毫克/千克的α-生育酚。
第2组的平均舍恩贝格评分、血清淀粉酶、脂肪酶和中性粒细胞明胶酶相关脂质运载蛋白（NGAL）水平在统计学上显著高于第1组。第3组的平均舍恩贝格评分以及血清淀粉酶和脂肪酶水平在统计学上显著低于第2组。
在本实验性研究的雨蛙素诱导的大鼠急性胰腺炎模型中，腹腔注射30毫克/千克的α-生育酚以检查其对胰腺炎的影响。在胰腺组织的组织病理学检查中观察到了改善。我们认为α-生育酚可能对胰腺组织有治疗作用。
