Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa, 16059, Turkey.
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Uludag University, Gorukle, Bursa, 16059, Turkey.
Cryobiology. 2021 Dec;103:45-48. doi: 10.1016/j.cryobiol.2021.09.012. Epub 2021 Oct 26.
The objective of the current study was to evaluate the effect of autologous platelet-rich plasma (PRP) addition into soybean lecithin based extender on buck semen at post-thaw. Semen samples were collected from eight Saanen buck, and each semen sample was split into four equal aliquots and diluted with different concentrations of PRP supplemented extenders [no PRP (control), 0.5 × 10/ml PRP, 1 × 10/ml PRP, or 2 × 10/ml PRP]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed at post-thaw. The results showed that 2 × 10/ml PRP group had a positive effect on motility (62.41 ± 4.24), membrane functional integrity (71.11 ± 2.90), mitochondrial membrane potential (69.70 ± 1.99), DNA integrity (7.22 ± 0.93) and MDA levels (2.56 ± 0.73) at post-thaw (P < 0.05). The results of the study demonstrated that autologous PRP has a protective effect on cryopreservation of buck spermatozoa and the fertility effects are worthy of further study.
本研究的目的是评估在解冻后将自体富血小板血浆（PRP）添加到大豆卵磷脂基础稀释液中对雄鹿精液的影响。从 8 只 Saanen 雄鹿中收集精液样本，每个精液样本均分为 4 等份，并分别用不同浓度添加 PRP 的稀释液稀释[无 PRP（对照）、0.5×10/ml PRP、1×10/ml PRP 或 2×10/ml PRP]。在解冻后对活力、质膜功能完整性、顶体完整性、线粒体膜电位、DNA 完整性和丙二醛浓度（MDA）进行测量和分析。结果表明，2×10/ml PRP 组对活力（62.41±4.24）、膜功能完整性（71.11±2.90）、线粒体膜电位（69.70±1.99）、DNA 完整性（7.22±0.93）和 MDA 水平（2.56±0.73）解冻后有积极影响（P<0.05）。研究结果表明，自体 PRP 对雄鹿精子冷冻保存具有保护作用，其生育效果值得进一步研究。
