Reproductive Medicine Center, Gansu Provincial Maternity and Child-Care Hospital, Lanzhou, China.
Department of Reproductive Medicine, Central Hospital Affiliated to Shandong First Medical University, Jinan, China; Department of Reproductive Medicine, Jinan Central Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Cryobiology. 2021 Feb;98:12-16. doi: 10.1016/j.cryobiol.2021.01.009. Epub 2021 Jan 19.
As a source of growth factors, platelet-rich plasma (PRP) has been widely used in the repair of various injuries due to its cytoprotective properties in regenerative medicine. The objective of the present study was to investigate the effect of autologous PRP supplementation on the quality of frozen-thawed human sperm. Twelve normozoospermic semen samples were collected, and each sample was divided into 4 aliquots and added with different proportions of PRP (0%, 2%, 5%, and 10%) separately, followed by cryopreservation. Sperm motility, viability, membrane integrity, DNA fragmentation index (DFI), reactive oxygen species (ROS) levels and mitochondrial membrane potential were measured and analyzed. The results showed that the addition of 5% PRP improved sperm progressive motility (30.3 ± 2.7 VS. 28.1 ± 2.6), viability (65.5 ± 4.2 VS. 59.6 ± 3.9), and membrane integrity (52.4 ± 3.6 VS. 49.2 ± 3.4) after cryopreservation (P < 0.05). Slight attenuation of ROS generation, restoration of mitochondrial membrane potential and reduction of DNA fragments were also observed in post-thawed sperm supplemented with PRP, but there was no significant (P > 0.05). In conclusion, autologous PRP has a partial protective effect on cryopreservation of human spermatozoa, and the combined application with other high-efficiency cryoprotectant is worthy of further study.
作为生长因子的来源，富血小板血浆（PRP）因其在再生医学中的细胞保护特性而在各种损伤的修复中得到了广泛的应用。本研究旨在探讨自体 PRP 补充对冻融人精子质量的影响。收集了 12 例正常精子样本，每个样本分为 4 等份，并分别添加不同比例的 PRP（0%、2%、5%和 10%），然后进行冷冻保存。测量并分析精子活力、存活率、膜完整性、DNA 碎片指数（DFI）、活性氧（ROS）水平和线粒体膜电位。结果表明，添加 5%的 PRP 可提高精子的前向运动能力（30.3±2.7 VS. 28.1±2.6）、存活率（65.5±4.2 VS. 59.6±3.9）和膜完整性（52.4±3.6 VS. 49.2±3.4）（P<0.05）。在添加 PRP 的解冻后精子中，ROS 生成、线粒体膜电位恢复和 DNA 片段减少也有轻微减弱，但无统计学意义（P>0.05）。综上所述，自体 PRP 对人精子的冷冻保存有一定的保护作用，与其他高效冷冻保护剂联合应用值得进一步研究。
