Johnson D E
Ann N Y Acad Sci. 1986;483:241-4. doi: 10.1111/j.1749-6632.1986.tb34526.x.
The AEM is a powerful tool in biological research, capable of providing information simply not available by other means. The use of a field emission STEM for this application can lead to a significant improvement in spatial resolution in most cases now allowed by the quality of the specimen preparation but perhaps ultimately limited by the effects of radiation damage. Increased elemental sensitivity is at least possible in selected cases with electron energy-loss spectrometry, but fundamental aspects of ELS will probably confine its role to that of a limited complement to EDS. The considerable margin for improvement in sensitivity of the basic analytical technique means that the search for technological improvement will continue. Fortunately, however, current technology can also continue to answer important biological questions.
先进电子显微镜（AEM）是生物学研究中的一种强大工具，能够提供其他方法无法获得的信息。在这种应用中使用场发射扫描透射电子显微镜（STEM），在大多数情况下，现在可以因样品制备质量而使空间分辨率得到显著提高，但最终可能会受到辐射损伤效应的限制。在某些选定的情况下，通过电子能量损失谱（ELS）至少有可能提高元素灵敏度，但ELS的基本特性可能会将其作用限制为能量色散谱（EDS）的有限补充。基本分析技术在灵敏度方面仍有相当大的提升空间，这意味着对技术改进的探索将继续。然而，幸运的是，当前技术也能够继续回答重要的生物学问题。
