Wang Youyou, Ren Huijun, Pan Zhaohu, Liu Ben, Lin Fan
Department of Otorhinolaryngology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Taizhou, Zhejiang 317000, China.
Evid Based Complement Alternat Med. 2021 Oct 19;2021:2681683. doi: 10.1155/2021/2681683. eCollection 2021.
To investigate the expression of miR-338-3p in nasopharyngeal carcinoma (NPC) and its relationship with STAT3 mRNA expression as well as their relationship with clinical pathological parameters and prognosis of patients.
From September 2016 to September 2018, 71 patients with NPC were selected as the NPC group, and 71 samples of NPC tissues were collected during the operation. A total of 23 patients who underwent biopsy due to chronic nasopharyngitis were selected as the control group and 23 nasopharyngeal mucosal tissues were collected. The expressions of miR-338-3p and STAT3 mRNA in nasopharyngeal tissue of two groups were detected by real-time quantitative PCR, and the relationship between the two was analyzed. To collect clinical data of NPC patients and analyze the relationship between the expressions of miR-338-3p and STAT3 in NPC tissues and clinical pathological parameters of the patients, we followed up the patients with nasopharyngeal carcinoma for three years to observe the relationship between miR-338-3p, STAT3, and the prognosis of the patients.
The relative expression levels of miR-338-3p in nasopharyngeal tissues of the NPC group and the control group were 0.39 ± 0.05 and 1.01 ± 0.09, respectively ( < 0.05). The relative expression levels of STAT3 mRNA in nasopharyngeal tissues of the NPC group and the control group were 3.82 ± 0.21 and 1.04 ± 0.11, respectively ( > 0.05). miR-338-3p was negatively correlated with the relative expression of STAT3 mRNA in nasopharyngeal carcinoma ( = 0.038, > 0.05). The expression of miR-338-3p was related to the age of the patient, clinical TNM stage, T stage, and distant metastasis (all < 0.05). STAT3 expression was correlated with clinical TNM stage, T stage, and distant metastasis in our patient ( < 0.05). The expressions of miR-338-3p and STAT3 in nasopharyngeal carcinoma tissues from different gender, histological type, N stage, M stage, and degree of differentiation showed no statistical differences ( > 0.05). The survival rate of the group with low miR-338-3p expression was significantly lower than that of the group with high miR-338-3p expression ( > 0.05). The survival rate of patients with the high STAT3 expression group was significantly lower than that of patients with the low STAT3 expression group ( > 0.05).
There is a negative correlation between the low expression of miR-338-3p and the high expression of STAT3 in NPC, which are all related to the TNM stage, T stage, and prognosis of the patient.
探讨miR-338-3p在鼻咽癌（NPC）中的表达及其与信号转导和转录激活因子3（STAT3）mRNA表达的关系，以及它们与患者临床病理参数和预后的关系。
选取2016年9月至2018年9月期间71例NPC患者作为NPC组，术中采集71份NPC组织样本。选取23例因慢性鼻咽炎接受活检的患者作为对照组，采集23份鼻咽黏膜组织。采用实时定量PCR检测两组鼻咽组织中miR-338-3p和STAT3 mRNA的表达，并分析两者之间的关系。收集NPC患者的临床资料，分析NPC组织中miR-338-3p和STAT3的表达与患者临床病理参数的关系，对鼻咽癌患者进行为期三年的随访，观察miR-338-3p、STAT3与患者预后的关系。
NPC组和对照组鼻咽组织中miR-338-3p的相对表达水平分别为0.39±0.05和1.01±0.09（<0.05）。NPC组和对照组鼻咽组织中STAT3 mRNA的相对表达水平分别为3.82±0.21和1.04±0.11（>0.05）。miR-338-3p与鼻咽癌中STAT3 mRNA的相对表达呈负相关（=0.038，>0.05）。miR-338-3p的表达与患者年龄、临床TNM分期、T分期和远处转移有关（均<0.05）。STAT3表达与本研究患者的临床TNM分期、T分期和远处转移相关（<0.05）。miR-338-3p和STAT3在不同性别、组织学类型、N分期、M分期和分化程度的鼻咽癌组织中的表达无统计学差异（>0.05）。miR-338-3p低表达组的生存率明显低于miR-338-3p高表达组（>0.05）。STAT3高表达组患者的生存率明显低于STAT3低表达组患者（>0.05）。
NPC中miR-338-3p低表达与STAT3高表达呈负相关，二者均与患者的TNM分期、T分期及预后有关。
