Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, P.R. China.
School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou, P.R. China.
J Cell Biochem. 2018 Feb;119(2):1931-1941. doi: 10.1002/jcb.26354. Epub 2017 Sep 18.
The aim of this study was to investigate the correlation of expression of IGF1R-RACK1-STAT3 and Bcl-xl in nasopharyngeal carcinoma (NPC) with the clinicopathological features and the prognosis of NPC. Our study selected 215 NPC tissues and 178 chronic nasopharyngitis tissues (control group). Positive expression rates of IGF1R, RACK1, STAT3, and Bcl-xl were tested by immunohistochemical method, and expression of IGF1R, RACK1, STAT3, Bcl-xl, Bcl-2, and Bax by western blotting. Correlation of IGF1R, RACK1, STAT3, and Bcl-xl with the clinicopathological features of NPC was analyzed. The correlation among those four expression was analyzed by Spearman. The survival of NPC and independent factors of prognosis were tested by Kaplan-Meier and COX proportional hazards model respectively. The NPC group had higher positive expression rates of IGF1R, RACK1, STAT3, and Bcl-xl, and elevated expression of IGF1R, RACK1, STAT3, Bcl-xl, Bcl-2, and Bax. The lymph node metastasis (LNM) group had higher positive expression rates of IGF1R and RACK1 when compared with the non-LNM group. Patients with stage III and IV had higher positive expression rates of IGF1R, RACK1, STAT3, and Bcl-xl. There was positive correlation between expression of IGF1R and RACK1, STAT3. Such correlation was found between RACK1 and STAT3. Patients with negative expression of IGF1R, RACK1, STAT3, and Bcl-xl had higher survival rates. The risky factors of poor prognosis of NPC were positive expression of IGF1R, RACK1, STAT3 and Bcl-xl, and LNM. IGF1R-RACK1-STAT3 and Bcl-xl expression correlated with the clinicopathological features and poor prognosis of NPC.
本研究旨在探讨胰岛素样生长因子 1 受体（IGF1R）-受体结合蛋白 1（RACK1）-信号转导和转录激活因子 3（STAT3）和 B 细胞淋巴瘤/白血病-2 家族蛋白（Bcl-2）相关蛋白 X（Bcl-xl）在鼻咽癌（NPC）中的表达与 NPC 临床病理特征及预后的相关性。本研究选取 215 例 NPC 组织和 178 例慢性鼻咽炎组织（对照组），采用免疫组化法检测 IGF1R、RACK1、STAT3 和 Bcl-xl 的阳性表达率，采用 Western blot 法检测 IGF1R、RACK1、STAT3、Bcl-xl、Bcl-2 和 Bax 的表达。分析 IGF1R、RACK1、STAT3 和 Bcl-xl 与 NPC 临床病理特征的相关性，采用 Spearman 分析四者之间的相关性。采用 Kaplan-Meier 法和 COX 比例风险模型分别检测 NPC 患者的生存情况及预后的独立因素。NPC 组 IGF1R、RACK1、STAT3 和 Bcl-xl 的阳性表达率均较高，IGF1R、RACK1、STAT3、Bcl-xl、Bcl-2 和 Bax 的表达水平均升高。与无淋巴结转移（LNM）组相比，有 LNM 组的 IGF1R 和 RACK1 阳性表达率较高。Ⅲ期和Ⅳ期患者的 IGF1R、RACK1、STAT3 和 Bcl-xl 阳性表达率较高。IGF1R 与 RACK1、STAT3 的表达呈正相关，RACK1 与 STAT3 的表达呈正相关。IGF1R、RACK1、STAT3 和 Bcl-xl 阴性表达的患者生存率较高。NPC 预后不良的危险因素为 IGF1R、RACK1、STAT3 和 Bcl-xl 阳性表达及 LNM。IGF1R-RACK1-STAT3 和 Bcl-xl 的表达与 NPC 的临床病理特征和不良预后相关。
