Hatatian Niayesh, Bosstani Reza, Mohammadi Asadollah, Mehraban Saeedeh, Mahdifar Maryam, Zemorshidi Fariba, Mozhgani Sayed-Hamidreza, Haji Ghadimi Ali, Foroughipour Mohsen, Rafatpanah Houshang
Immunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Neurology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Basic Med Sci. 2021 Jul;24(7):992-996. doi: 10.22038/ijbms.2021.50821.11569.
HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a neuroinflammatory disorder associated with HTLV-1. Cytokines and inflammatory mediators have a major role in forming inflammation in HAM/TSP patients. This study aimed to measure the levels of IL-32, a proinflammatory cytokine associated with autoinflammatory disorders, and also cyclooxygenase -2 (COX-2) as a key mediator of inflammatory pathways in HAM/TSP patients and HTLV-1 asymptomatic carriers (ACs).
Peripheral blood monocyte cells (PBMCs) were isolated from HAM/TSP patients, ACs, and healthy controls (HCs), and DNA and RNA were extracted to evaluate HTLV-1 proviral load (PVL) and expression of IL-32 and COX-2, using real-time PCR. Serum levels of IL-32 were determined by using an ELISA assay.
The expression level of IL-32 was significantly higher in ACs compared with HAM/TSP patients and HCs (<0.0001 and >0.05, respectively). There were no statistically significant differences in the expression levels of Cox-2 and protein levels of IL-32 between the study groups. HTLV-1 PVL was higher in HAM/TSP patients compared with ACs.
Results showed increased mRNA levels of IL-32 in ACs. Since HTLV-1 PVL in ACs is lower than in HAM/TSP patients, it could be concluded that IL-32 might be an HTLV-1 inhibitor that seems to control virus replication. Despite the difference in IL-32 mRNA levels between study groups, no statistically significant differences were observed in IL-32 serum levels. Also, there were no significant differences in COX-2 expression.
人类嗜T淋巴细胞病毒1型（HTLV-1）相关脊髓病/热带痉挛性截瘫（HAM/TSP）是一种与HTLV-1相关的神经炎症性疾病。细胞因子和炎症介质在HAM/TSP患者炎症形成中起主要作用。本研究旨在检测白细胞介素32（IL-32，一种与自身炎症性疾病相关的促炎细胞因子）以及环氧化酶-2（COX-2，作为HAM/TSP患者和HTLV-1无症状携带者（ACs）炎症途径的关键介质）的水平。
从HAM/TSP患者、ACs和健康对照（HCs）中分离外周血单个核细胞（PBMCs），提取DNA和RNA，采用实时聚合酶链反应（PCR）评估HTLV-1前病毒载量（PVL）以及IL-32和COX-2的表达。采用酶联免疫吸附测定（ELISA）法测定血清IL-32水平。
与HAM/TSP患者和HCs相比，ACs中IL-32的表达水平显著更高（分别<0.0001和>0.05）。研究组之间Cox-2的表达水平和IL-32的蛋白水平无统计学显著差异。与ACs相比，HAM/TSP患者的HTLV-1 PVL更高。
结果显示ACs中IL-32的mRNA水平升高。由于ACs中的HTLV-1 PVL低于HAM/TSP患者，因此可以得出结论，IL-32可能是一种HTLV-1抑制剂，似乎可控制病毒复制。尽管研究组之间IL-32 mRNA水平存在差异，但IL-32血清水平未观察到统计学显著差异。此外，COX-2表达也无显著差异。
