Zin Noraziah M, Ismail Aishah, Mark David R, Westrop Gareth, Schniete Jana K, Herron Paul R
School of Diagnostic and Applied Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom.
Front Bioeng Biotechnol. 2021 Oct 12;9:740722. doi: 10.3389/fbioe.2021.740722. eCollection 2021.
Endophytic actinobacteria offer great potential as a source of novel bioactive compounds. In order to investigate the potential for the production of secondary metabolites by endophytes, we recovered a filamentous microorgansism from the tree Miq. After phenotypic analysis and whole genome sequencing we demonstrated that this organism, SUK42 was a member of the actinobacterial genus . This strain has a small genome in comparison with other type strains of this genus and has lost metabolic pathways associated with Stress Response, Nitrogen Metabolism and Secondary Metabolism. Despite this SUK42 can grow well in a laboratory environment and encodes a core genome that is consistent with other members of the genus. Finally, in contrast to other members of , SUK42 encodes saccharide secondary metabolite biosynthetic gene clusters, one of which with similarity to the acarviostatin cluster, the product of which displays α-amylase inhibitory activity. As extracts of the host plant demonstrate this inhibitory activity, it suggests that the potential medicinal properties of Miq might be provided by the endophytic partner and illustrate the potential for exploitation of endophytes for clinical or industrial uses.
内生放线菌作为新型生物活性化合物的来源具有巨大潜力。为了研究内生菌产生次生代谢物的潜力，我们从米槠树中分离出一种丝状微生物。经过表型分析和全基因组测序，我们证明该微生物SUK42是放线菌属的成员。与该属的其他模式菌株相比，该菌株的基因组较小，并且失去了与应激反应、氮代谢和次生代谢相关的代谢途径。尽管如此，SUK42仍能在实验室环境中良好生长，并且编码一个与该属其他成员一致的核心基因组。最后，与该属的其他成员不同，SUK42编码糖类次生代谢物生物合成基因簇，其中一个与阿卡拉他汀簇相似，其产物具有α-淀粉酶抑制活性。由于宿主植物的提取物显示出这种抑制活性，这表明米槠的潜在药用特性可能由内生共生伙伴提供，并说明了利用内生菌用于临床或工业用途的潜力。
