Metabolite profiling of endophytic spp. and its antiplasmodial potential.

BACKGROUND

Antiplasmodial drug discovery is significant especially from natural sources such as plant bacteria. This research aimed to determine antiplasmodial metabolites of spp. against 3D7 by using a metabolomics approach.

METHODS

strains' growth curves, namely SUK 12 and SUK 48, were measured and . 3D7 IC values were calculated. Metabolomics analysis was conducted on both strains' mid-exponential and stationary phase extracts.

RESULTS

The most successful antiplasmodial activity of SUK 12 and SUK 48 extracts shown to be at the stationary phase with IC values of 0.8168 ng/mL and 0.1963 ng/mL, respectively. In contrast, the IC value of chloroquine diphosphate (CQ) for antiplasmodial activity was 0.2812 ng/mL. The univariate analysis revealed that 854 metabolites and 14, 44 and three metabolites showed significant differences in terms of strain, fermentation phase, and their interactions. Orthogonal partial least square-discriminant analysis and S-loading plot putatively identified pavettine, aurantioclavine, and 4-butyldiphenylmethane as significant outliers from the stationary phase of SUK 48. For potential isolation, metabolomics approach may be used as a preliminary approach to rapidly track and identify the presence of antimalarial metabolites before any isolation and purification can be done.