Department of Animal Production, Faculty of Agriculture and Environmental Studies, Université Evangélique en Afrique (UEA), Bukavu, DR Congo.
Department of Veterinary Anatomy and Physiology, University of Nairobi, Nairobi, Kenya.
Vet Med Sci. 2021 May;7(3):1047-1055. doi: 10.1002/vms3.443. Epub 2021 Feb 8.
Glyphosate formulations, widely applied non-selective systemic herbicides, are progressively becoming the most controversial pesticides on the market due the adverse effects they pose to humans and environment. The information on these potential hazardous effects to the handlers of the pesticide remains obscure. This study investigated effects of glyphosate-based herbicide on growth performance, seminal parameters and hemato-biochemical profiles in male guinea pig. Forty sexually mature male guinea pigs weighing between 393.3 and 418.4 g were divided into four groups of 10 animals each and orally administered 0, 186, 280 and 560 mg/kg body weight of WILLOSATE daily for 60 days. Daily feed intake and body weight gain were recorded. At the end of experimental period all animals were humanely sacrificed, and blood samples and vital organs were collected for appropriate analysis. Results showed a significant decrease (p < 0.05) in body weight gain (-102.2%), final body weight (-9.8%) and feed intake (-13.1%) of animals following sub-chronic exposure of WILLOSATE. The weights of the liver and kidney increased significantly (p < 0.05) by 25.4% and 28.8%, respectively, while testicular weights decreased (p < 0.05) by 24% with increasing doses of WILLOSATE. A decrease in sperm motility (-67.9%), viability (-52.7%) and concentration per vas deferens (-40.7%), and an increase in sperm major (28.1%) and minor (45.3%) morphological aberrations were recorded in WILLOSATE - exposed guinea pigs when compared to controls. There was a dose-dependent increase (p < 0.05) in MCV and WBC and a decrease in Hb content and RBC, as well as serum content in total protein (-11.8%). The serum content of cholesterol (37.8%), urea (87.1%), creatinine (22.4%), ALAT (74.2%) and ASAT (88.7%) were significantly higher in treated groups compared to controls. These results point toward the toxic effects of WILLOSATE on vital organs and reproductive function of the body at high doses and long-term exposure.
草甘膦制剂是一种广泛应用的非选择性系统性除草剂，由于其对人类和环境造成的不良影响，逐渐成为市场上最具争议的农药。然而，关于这些潜在危险对农药处理人员的影响的信息仍然不清楚。本研究调查了草甘膦除草剂对雄性豚鼠生长性能、精液参数和血液生化特征的影响。将 40 只性成熟雄性豚鼠按体重分为 4 组，每组 10 只，分别每天口服 0、186、280 和 560mg/kg 体重的 WILLOSATE，连续 60 天。记录每日采食量和体重增加。实验期末，所有动物均人道处死，采集血液样本和重要器官进行适当分析。结果表明，WILLOSATE 亚慢性暴露后，动物体重增加（-102.2%）、终体重（-9.8%）和采食量（-13.1%）显著下降（p<0.05）。肝、肾重量分别显著增加（p<0.05）25.4%和 28.8%，而睾丸重量则因 WILLOSATE 剂量增加而显著降低（p<0.05），降低了 24%。与对照组相比，WILLOSATE 暴露的豚鼠精子活力（-67.9%）、活力（-52.7%）和每输精道浓度（-40.7%）下降，精子大头（28.1%）和小头（45.3%）形态异常增加。MCV 和 WBC 呈剂量依赖性增加（p<0.05），Hb 含量和 RBC 以及血清总蛋白含量（-11.8%）降低。与对照组相比，血清胆固醇（37.8%）、尿素（87.1%）、肌酐（22.4%）、丙氨酸氨基转移酶（74.2%）和天冬氨酸氨基转移酶（88.7%）含量显著升高。这些结果表明，WILLOSATE 在高剂量和长期暴露下对重要器官和身体生殖功能具有毒性作用。
