Dieter M P, Maronpot R R, French J E
Cancer Detect Prev. 1987;10(5-6):425-33.
Cellular glucose-metabolizing enzymes and acetylcholinesterase (AChE) have been utilized as biochemical markers of mononuclear cell (MNC) leukemia maintained by serial cell transplantation in F344 rats. We have evaluated the sensitivity and reproducibility of these tumor markers in comparison to other diagnostic criteria of leukemia. Weanling rats were injected with 2 X 10(7) leukemic spleen MNC and sampled at 6, 35, 63, and 83 days. At 6 days, the glycolytic enzyme activities from spleen that decreased were believed to be residual activity from injected leukemic MNC. Glycolytic enzyme activities in spleen MNC were normal at 35 days and no changes in blood MNC enzyme activity occurred at 6 days or 35 days. At 63 days, prior to clinical evidence of leukemia, glucose-metabolizing enzymes from spleen MNC changed, and there were decreases in AChE from both blood and spleen MNC that progressively decreased at 83 days, when there was depressed body weight, splenomegaly, elevated WBC, depressed RBC, hypoglycemia, hyperbilirubinemia, and elevated serum enzyme levels. Separation of leukemic MNC from blood and spleen enhances sensitivity of cellular enzyme responses and provides a reproducible model to study biochemical markers correlated with severity of leukemia.
细胞葡萄糖代谢酶和乙酰胆碱酯酶（AChE）已被用作通过在F344大鼠中进行连续细胞移植维持的单核细胞（MNC）白血病的生化标志物。与白血病的其他诊断标准相比，我们评估了这些肿瘤标志物的敏感性和可重复性。给断乳大鼠注射2×10⁷白血病脾脏MNC，并在第6、35、63和83天取样。在第6天，脾脏中降低的糖酵解酶活性被认为是注射的白血病MNC的残留活性。脾脏MNC中的糖酵解酶活性在第35天正常，血液MNC酶活性在第6天或第35天没有变化。在第63天，在白血病的临床证据出现之前，脾脏MNC的葡萄糖代谢酶发生了变化，血液和脾脏MNC中的AChE均降低，在第83天逐渐下降，此时体重下降、脾肿大、白细胞升高、红细胞降低、低血糖、高胆红素血症和血清酶水平升高。从血液和脾脏中分离白血病MNC可提高细胞酶反应的敏感性，并提供一个可重复的模型来研究与白血病严重程度相关的生化标志物。
