Dieter M P, Maronpot R R, French J E
Cancer Res. 1985 Sep;45(9):4301-7.
Mononuclear cell (MNC) leukemia was identified in 26-month-old F344 rats by splenomegaly, reduced red blood cell counts, and elevated white blood cell counts. Atypical MNC were predominant in spleen and blood with acentric nuclei and red cytoplasmic granules. Pentose shunt, glycolytic, and Krebs cycle enzyme activities were elevated 2- to 11-fold in the enriched MNC fraction (Ficoll-Paque density gradients, 1.077 g/ml) isolated from spleen. A leukemic MNC line was derived from one of the spontaneously leukemic donors and then maintained in vivo by s.c. transfer of 2 X 10(7) spleen cells into 7-8-week-old syngeneic recipients. In these serial transplantation experiments leukemia that was clinically and morphologically indistinguishable from spontaneous leukemia in 104-week-old rats was induced in 22-24-week-old rats. Enhanced enzyme activity in MNC was not essential to maintain the phenotypic expression of Fischer rat leukemia. The pattern of biochemical response in spleen MNC from transplanted cases was the opposite of that previously noted in spontaneously leukemic rats, with 50-70% decreases in the specific activities of pentose shunt enzymes and malate dehydrogenase. Reversal of the expression of these enzymes in MNC may be related to a difference in the growth rate of the tumors or to selective proliferation of the transplanted leukemic cells. In addition acetylcholinesterase activity decreased 35-85% in MNC of spleen and blood. Transplantable MNC from F344 rats provide abundant tumorigenic material with a novel biochemical expression that may be useful in the study of chemotherapeutic intervention.
通过脾肿大、红细胞计数减少和白细胞计数升高，在26月龄的F344大鼠中鉴定出单核细胞（MNC）白血病。非典型MNC在脾脏和血液中占主导地位，具有偏心核和红色细胞质颗粒。从脾脏分离的富集MNC部分（Ficoll-Paque密度梯度，1.077 g/ml）中，戊糖分流、糖酵解和三羧酸循环酶活性提高了2至11倍。从一只自发白血病供体中获得了白血病MNC系，然后通过将2×10⁷个脾细胞皮下转移到7至8周龄的同基因受体中在体内维持。在这些连续移植实验中，22至24周龄的大鼠诱发了白血病，其临床和形态学与104周龄大鼠的自发白血病无法区分。MNC中增强的酶活性对于维持Fischer大鼠白血病的表型表达并非必不可少。移植病例脾脏MNC中的生化反应模式与先前在自发白血病大鼠中观察到的相反，戊糖分流酶和苹果酸脱氢酶的比活性降低了50%至70%。MNC中这些酶表达的逆转可能与肿瘤生长速率的差异或移植白血病细胞的选择性增殖有关。此外，脾脏和血液MNC中的乙酰胆碱酯酶活性降低了35%至85%。来自F344大鼠的可移植MNC提供了丰富的致瘤物质，具有新的生化表达，可能有助于化疗干预的研究。
