Merrick B A, He C, Dieter M P, Selkirk J K
Chemical Carcinogenesis Mechanisms Group, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Appl Theor Electrophor. 1993;3(5):203-11.
Proteins from leukemic spleen cells derived from a F344 rat transplant model were investigated by 2D gel electrophoresis. The cell line was maintained in vivo by serial transplant of mononuclear spleen cells from leukemic donors into syngeneic recipients. Cytosolic proteins from mononuclear cells (MNC) were isolated from spleens of normal and leukemic rats and separated by electrophoresis. Replicate data from silver-stained gels for each preparation were compiled into Master Images using image analysis algorithms in order to characterize the normal and leukemic protein profiles. Comparative analysis showed a total of 458 proteins that were reproducibly detected in normal MNC, while almost twice the number of proteins (828) were found in leukemic MNC, suggestive of the more mitotically active tumor cells. Profile analysis showed that normal and leukemic preparations shared 228 common proteins, with 600 proteins observed only in leukemic MNC, and 230 proteins found only in normal MNC. Differences in protein patterns between normal and leukemic MNC in rats probably reflects a shift in spleen leukocyte populations and a relative induction of gene expression in leukemic MNC as compared to the normal MNC.
采用二维凝胶电泳对源自F344大鼠移植模型的白血病脾细胞中的蛋白质进行了研究。通过将白血病供体的单核脾细胞连续移植到同基因受体体内来维持该细胞系在体内的生长。从正常大鼠和白血病大鼠的脾脏中分离出单核细胞（MNC）的胞质蛋白，并进行电泳分离。使用图像分析算法将每种制剂的银染凝胶的重复数据汇编成主图像，以表征正常和白血病蛋白质谱。比较分析显示，在正常MNC中可重复检测到总共458种蛋白质，而在白血病MNC中发现的蛋白质数量几乎是其两倍（828种），这表明肿瘤细胞的有丝分裂活性更高。图谱分析表明，正常制剂和白血病制剂共有228种共同蛋白质，其中600种蛋白质仅在白血病MNC中观察到，230种蛋白质仅在正常MNC中发现。大鼠正常MNC和白血病MNC之间蛋白质模式的差异可能反映了脾白细胞群体的变化以及与正常MNC相比白血病MNC中基因表达的相对诱导。
