UFRGS, Universidade Federal do Rio Grande do Sul, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.
INSEMINE, Human Reproductive Center, Porto Alegre, Brazil.
JBRA Assist Reprod. 2022 Aug 4;26(3):422-425. doi: 10.5935/1518-0557.20210090.
Endometriosis is associated with infertility, even without an anatomical abnormality. Furthermore, the peritoneal (mild) phenotype of this disease is the most prevalent and linked to infertility. The present study aimed to investigate the p63 gene and protein expression in granulosa cells from pre-ovulatory follicles in patients with endometriosis and infertility submitted to in vitro fertilization.
Twenty-eight patients participated in the study and were divided into two groups according to the presence or absence of endometriosis. The p63 gene-expression levels assessment was performed by real-time PCR (qPCR) using the TaqMan assay, and we used immunofluorescence to check the p63 protein expression after IVF.
There was no significant difference between the groups regarding age, hormonal levels, oocyte standards, and p63 gene expression. The control group showed an RQ of 1.000 (0.431 to 2.323) and the study group showed an RQ of 0.725 (0.249 to 2.105), p>0.05. Both groups showed a weak expression of the p63 gene (p>0.05).
This study described that endometriosis may not affect the p63 gene expression. Moreover, after follicular recruitment and growth, we found a weak expression of this protein, suggesting it is not part of oocyte maturation and development control.
子宫内膜异位症与不孕有关，即使没有解剖学异常也是如此。此外，这种疾病的腹膜（轻度）表型最为普遍，与不孕有关。本研究旨在研究子宫内膜异位症和不孕患者接受体外受精时的促黄体生成素峰前卵泡颗粒细胞中 p63 基因和蛋白的表达。
28 名患者参与了本研究，并根据是否存在子宫内膜异位症分为两组。采用 TaqMan 检测法通过实时 PCR（qPCR）评估 p63 基因表达水平，并用免疫荧光法检查 IVF 后的 p63 蛋白表达。
两组在年龄、激素水平、卵母细胞标准和 p63 基因表达方面无显著差异。对照组的 RQ 为 1.000（0.431 至 2.323），研究组的 RQ 为 0.725（0.249 至 2.105），p>0.05。两组均显示 p63 基因表达较弱（p>0.05）。
本研究表明子宫内膜异位症可能不会影响 p63 基因的表达。此外，在卵泡募集和生长后，我们发现这种蛋白表达较弱，表明它不是卵母细胞成熟和发育调控的一部分。
