Singh V K, Yamaki K, Donoso L A, Shinohara T
Laboratory of Immunology, National Eye Institute, Bethesda, MD 20892.
Curr Eye Res. 1988 Jan;7(1):87-92. doi: 10.3109/02713688809047025.
Experimental autoimmune uveitis (EAU) was observed in Hartley guinea pigs following immunization with two small synthetic peptides, peptide M and peptide M15L, which correspond to the amino acid sequence of a well-characterized region of bovine S-antigen. Groups of guinea pigs were immunized with 100 micrograms of each peptide in complete Freund's adjuvant and examined at regular intervals for the development of disease. Approximately two weeks later, an EAU was present which was characterized clinically by iris and pericorneal hyperemia. Histopathologically, a severe inflammatory response involving the uveal tract and retina was observed. In these eyes the photoreceptor cell layer of the retina was destroyed. A subretinal exudate containing mononuclear cells and polymorphonuclear leukocytes was also present. In addition, animals with EAU showed an associated pinealitis characterized by a lymphocytic infiltration of the subcapsular and central area of the pineal gland. Furthermore, draining lymph node cells of guinea pigs immunized with peptide M showed strong in vitro proliferative responses towards peptide M as measured by 3H thymidine uptake. These results demonstrate the existence of at least one common pathogenic epitope in bovine S-antigen for the induction of EAU in Hartley guinea pigs and Lewis rats.
在用两种小合成肽（肽M和肽M15L）免疫Hartley豚鼠后观察到实验性自身免疫性葡萄膜炎（EAU），这两种肽对应于牛S抗原一个特征明确区域的氨基酸序列。将豚鼠分组，用100微克每种肽与完全弗氏佐剂混合进行免疫，并定期检查疾病的发展情况。大约两周后，出现了EAU，其临床特征为虹膜和角膜周围充血。组织病理学上，观察到涉及葡萄膜和视网膜的严重炎症反应。在这些眼中，视网膜的光感受器细胞层被破坏。还存在含有单核细胞和多形核白细胞的视网膜下渗出物。此外，患有EAU的动物表现出相关的松体炎，其特征是松果体被膜下和中心区域有淋巴细胞浸润。此外，用肽M免疫的豚鼠引流淋巴结细胞对肽M表现出强烈的体外增殖反应，通过3H胸苷摄取来测量。这些结果证明，在牛S抗原中存在至少一个共同的致病表位，可在Hartley豚鼠和Lewis大鼠中诱导EAU。
