International Research Centre for Nano Handling and Manufacturing of China (CNM), Changchun University of Science and Technology, Changchun, 130022, China.
Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun, 130022, China.
Appl Biochem Biotechnol. 2022 Mar;194(3):1359-1372. doi: 10.1007/s12010-021-03663-0. Epub 2021 Oct 29.
In this study, the bi-layered disulfiram-loaded fiber membranes with the antibacterial activity and different surface wettabilities are prepared using electrospinning technology. In the application of wound dressing, the hydrophilic surface of fiber membranes is beneficial for cell adhesion and drug release to heal the wound. Meanwhile, the outside hydrophobic surface is able to block water penetration to reduce the probability of wound infection. The obtained bi-layered drug-loaded fiber membranes are composed of polyvinylidene fluoride (PVDF) bottom surface and disulfiram (DSF)/polylactic acid (PLA) top surface. To modify the top surface wettability, the oxygen plasma modification of bi-layered membranes was carried out. The morphology, wettability, and chemical compositions of bi-layered drug-loaded fiber membranes were analyzed using the scanning electronic microscope (SEM), drop shape analysis instrument, X-ray diffractometer (XRD), and X-ray photoelectron spectrometer (XPS). The bi-layered disulfiram-loaded membranes showed the potent antibacterial activity in vitro against both Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). It was found that the bi-layered membranes had good biocompatibility with L929 cells. Thus, the obtained bi-layered disulfiram-loaded fiber membranes are suitable for wound dressing application.
本研究采用静电纺丝技术制备了具有抗菌活性和不同表面润湿性的双层载双硫仑纤维膜。在伤口敷料的应用中，纤维膜的亲水表面有利于细胞黏附和药物释放，从而促进伤口愈合。同时，外部疏水面能够阻止水分渗透，降低伤口感染的概率。所得双层载药纤维膜由聚偏氟乙烯（PVDF）底层和载双硫仑（DSF）/聚乳酸（PLA）顶层组成。为了修饰顶层的润湿性，对双层膜进行了氧等离子体改性。采用扫描电子显微镜（SEM）、液滴形状分析仪器、X 射线衍射仪（XRD）和 X 射线光电子能谱仪（XPS）对双层载药纤维膜的形貌、润湿性和化学组成进行了分析。双层载双硫仑膜在体外对大肠杆菌（革兰氏阴性）和金黄色葡萄球菌（革兰氏阳性）均表现出很强的抗菌活性。结果表明，双层膜与 L929 细胞具有良好的生物相容性。因此，所得到的双层载双硫仑纤维膜适用于伤口敷料应用。
