Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Rio Ebro-Edificio I+D, C/Mariano Esquillor S/N, 50018 Zaragoza, Spain; Aragon Health Research Institute (IIS Aragon), 50009 Zaragoza, Spain.
LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
Mater Sci Eng C Mater Biol Appl. 2019 Oct;103:109822. doi: 10.1016/j.msec.2019.109822. Epub 2019 May 29.
To accomplish a rapid wound healing it is necessary to develop an asymmetric membrane with interconnected pores consisting of a top layer that prevents rapid dehydration of the wound and bacteria penetration and a sub-layer with high absorption capacity and bactericidal properties. Polycaprolactone (PCL)/polyvinyl acetate (PVAc) asymmetric membranes loaded with the bactericidal monoterpene carvacrol (CRV) were synthesized and characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. Mechanical properties in dry and wet conditions and fluid handling behavior were also assessed. In addition, biological studies regarding their bactericidal effects, cytocompatibility and wound closure properties were also developed. Loading efficiencies of 40-50% were achieved in the prepared samples and 85-100% of the loaded CRV was released in simulated wound pH evolution medium. The significant inhibition of Gram negative (Escherichia coli S17) and Gram positive (Staphylococcus aureus ATCC 25923) bacteria growth clearly showed the suitability of the fabricated membranes for wound healing applications. Furthermore, cytocompatibility of the loaded membranes was demonstrated both in 2D and 3D human dermal fibroblast cultures, as well as cell migration was not impaired by released carvacrol from the membranes. These results highlight the potential of these polymeric electrospun membranes for wound healing.
为了实现快速伤口愈合，有必要开发一种具有互连通孔的不对称膜，该膜由防止伤口快速脱水和细菌渗透的顶层和具有高吸收能力和杀菌性能的亚层组成。本文制备了负载杀菌单萜香芹酚（CRV）的聚己内酯（PCL）/聚醋酸乙烯酯（PVAc）不对称膜，并通过扫描电子显微镜和傅里叶变换红外光谱对其进行了表征。还评估了其在干燥和湿润条件下的机械性能以及流体处理性能。此外，还研究了其杀菌效果、细胞相容性和伤口闭合性能等生物学性能。在制备的样品中，负载效率达到了 40-50%，在模拟伤口 pH 演变介质中，负载的 CRV 有 85-100%被释放。负载的 CRV 对革兰氏阴性（大肠杆菌 S17）和革兰氏阳性（金黄色葡萄球菌 ATCC 25923）细菌的显著抑制作用清楚地表明了所制备的膜在伤口愈合应用中的适用性。此外，负载膜在 2D 和 3D 人真皮成纤维细胞培养中表现出良好的细胞相容性，并且从膜中释放的香芹酚不会损害细胞迁移。这些结果突出了这些聚合物电纺膜在伤口愈合中的潜力。
