E 型淋球菌沙眼衣原体在男男性行为者中的克隆种系传播。
Spread of clonal genovar E Chlamydia trachomatis among men who have sex with men.
机构信息
Department of Bacteriology, National Reference Centre for Bacterial Sexually Transmitted Infections, CHU Bordeaux, Bordeaux, France.
Section of Clinical Bacteriology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
出版信息
PLoS One. 2021 Oct 29;16(10):e0259274. doi: 10.1371/journal.pone.0259274. eCollection 2021.
In a previous study, we developed a Multi-Locus VNTRs Analysis (MLVA) typing system, called MLVA-5, for the discrimination of Chlamydia trachomatis genovar E strain. The results suggested the clonal spread of a MLVA-5 type 21 strain among men who have sex with men (MSM). We applied the MLVA-5 typing method on 157 French anorectal genovar E specimens and 19 Swedish specimens collected between 2010 and 2015. A total of 29 MLVA-5 types was obtained, with three predominant types among French samples: 78 specimens belonged to MLVA-5 type 21, two other types, 11 and 13, included 9 and 14 specimens, respectively. In 15 cases, one unique MLVA-5 type was observed for a single patient, 7 of which were new types not previously described. The distribution of MLVA-5 types according to sexual orientation showed that the 7 anorectal specimens from heterosexual patients belonged to 6 genotypes, and the 12 anorectal specimens from bisexual patients comprised eight types. The 95 anorectal specimens from MSM were distributed into 22 types, but 55 (57.9%) of them belonged to MLVA-5 type 21. Among the Swedish specimens from MSM, eight were from MLVA-type 21 (4 urines and 4 anorectal specimens). The results support the hypothesis of the spread of clonal genovar E strain among MSM.
在之前的研究中，我们开发了一种多基因座可变数目串联重复序列分析（MLVA）分型系统，称为 MLVA-5，用于区分沙眼衣原体 E 变种菌株。结果表明，MLVA-5 型 21 株在男男性行为者（MSM）中存在克隆传播。我们在 157 例法国直肠 E 变种标本和 19 例 2010 年至 2015 年间采集的瑞典标本上应用了 MLVA-5 分型方法。总共获得了 29 种 MLVA-5 型，其中法国样本中有三种主要类型：78 例属于 MLVA-5 型 21，另外两种类型 11 和 13 分别包含 9 和 14 例。在 15 例中，每个患者观察到一种独特的 MLVA-5 型，其中 7 种是以前未描述的新型。根据性取向分布的 MLVA-5 型表明，7 例异性恋患者的直肠标本属于 6 种基因型，12 例双性恋患者的直肠标本包括 8 种类型。95 例 MSM 的直肠标本分为 22 种类型，但其中 55 种（57.9%）属于 MLVA-5 型 21。在 MSM 的瑞典标本中，有 8 例来自 MLVA-21 型（4 例尿液和 4 例直肠标本）。结果支持了 E 变种菌株在 MSM 中克隆传播的假说。
Zotero 插件