Department of Process Engineering and Chemical Technology, Faculty of Chemistry, Gdansk University of Technology, 11/12 Gabriela Narutowicza Street, 80-233 Gdansk, Poland.
Molecules. 2022 Nov 7;27(21):7658. doi: 10.3390/molecules27217658.
Using bioconversion and simultaneous value-added product generation requires purification of the gaseous and the liquid streams before, during, and after the bioconversion process. The effect of diversified process parameters on the efficiency of biohydrogen generation via biological processes is a broad object of research. Biomass-based raw materials are often applied in investigations regarding biohydrogen generation using dark fermentation and photo fermentation microorganisms. The literature lacks information regarding model mixtures of lignocellulose and starch-based biomass, while the research is carried out based on a single type of raw material. The utilization of lignocellulosic and starch biomasses as the substrates for bioconversion processes requires the decomposition of lignocellulosic polymers into hexoses and pentoses. Among the components of lignocelluloses, mainly lignin is responsible for biomass recalcitrance. The natural carbohydrate-lignin shields must be disrupted to enable lignin removal before biomass hydrolysis and fermentation. The matrix of chemical compounds resulting from this kind of pretreatment may significantly affect the efficiency of biotransformation processes. Therefore, the actual state of knowledge on the factors affecting the culture of dark fermentation and photo fermentation microorganisms and their adaptation to fermentation of hydrolysates obtained from biomass requires to be monitored and a state of the art regarding this topic shall become a contribution to the field of bioconversion processes and the management of liquid streams after fermentation. The future research direction should be recognized as striving to simplification of the procedure, applying the assumptions of the circular economy and the responsible generation of liquid and gas streams that can be used and purified without large energy expenditure. The optimization of pre-treatment steps is crucial for the latter stages of the procedure.
在生物转化过程之前、期间和之后，需要对气态和液态流进行纯化，以利用生物转化和同时生成增值产品。多样化的工艺参数对生物法产氢效率的影响是一个广泛的研究对象。生物基原料常用于暗发酵和光发酵微生物产氢的研究。文献中缺乏关于木质纤维素和淀粉基生物质模型混合物的信息，而研究是基于单一类型的原料进行的。木质纤维素和淀粉生物质作为生物转化过程的底物的利用需要将木质纤维素聚合物分解为己糖和戊糖。在木质纤维素的成分中，主要是木质素导致生物质的顽固性。在生物质水解和发酵之前，必须破坏天然碳水化合物-木质素的屏蔽，以去除木质素。这种预处理产生的化合物基质可能会显著影响生物转化过程的效率。因此，需要监测影响暗发酵和光发酵微生物培养及其对生物质水解产物发酵的适应的因素的实际知识状态，并成为生物转化过程和发酵后液体流管理领域的一个贡献。未来的研究方向应被视为努力简化该程序，应用循环经济的假设和负责任地生成可使用和净化而无需大量能源消耗的液体和气体流。预处理步骤的优化对于该程序的后期阶段至关重要。
