Henan Province Engineering Research Center for Forest Biomass Value-added Products, School of Forestry, Henan Agricultural University, Zhengzhou, Henan, 450002, China; Neuroinflammation Group, Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, NSW, Australia; Biofuel Research Team (BRTeam), Terengganu, Malaysia.
Neuroinflammation Group, Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, NSW, Australia; Biofuel Research Team (BRTeam), Terengganu, Malaysia; PANDIS.org, Australia.
Bioresour Technol. 2022 Jan;344(Pt A):126212. doi: 10.1016/j.biortech.2021.126212. Epub 2021 Oct 27.
Appropriate bioprocessing of lignocellulosic materials into ethanol could address the world's insatiable appetite for energy while mitigating greenhouse gases. Bioethanol is an ideal gasoline extender and is widely used in many countries in blended form with gasoline at specific ratios to improve fuel characteristics and engine performance. Although the bioethanol production industry has long been operational, finding a suitable microbial agent for the efficient conversion of lignocelluloses is still an active field of study. Among available microbial candidates, engineered bacteria may be promising ethanol producers while may show other desired traits such as thermophilic nature and high ethanol tolerance. This review provides the current knowledge on the introduction, overexpression, and deletion of the genes that have been performed in bacterial hosts to achieve higher ethanol yield, production rate and titer, and tolerance. The constraints and possible solutions and economic feasibility of the processes utilizing such engineered strains are also discussed.
将木质纤维素材料进行适当的生物加工转化为乙醇，可以满足世界对能源的无尽需求，同时减少温室气体排放。生物乙醇是一种理想的汽油增溶剂，在许多国家广泛以特定比例与汽油混合使用，以改善燃料特性和发动机性能。尽管生物乙醇生产行业已经存在很长时间，但寻找合适的微生物剂来高效转化木质纤维素仍然是一个活跃的研究领域。在可用的微生物候选物中，经过工程改造的细菌可能是有前途的乙醇生产者，同时可能表现出其他理想的特性，如嗜热性质和高乙醇耐受性。本文综述了在细菌宿主中进行的基因导入、过表达和缺失，以提高乙醇产量、生产速率和浓度以及耐受性的最新知识。还讨论了利用这些工程菌株的过程中的限制因素以及可能的解决方案和经济可行性。
