Department of Electrical Engineering, Santa Catarina State University, Joinville 89219-710, Brazil.
Rev Sci Instrum. 2021 Oct 1;92(10):104704. doi: 10.1063/5.0053707.
Human cell cultures are powerful laboratory tools for biological models of diseases, drug development, and tissue engineering. However, the success of biological experiments often depends on real-time monitoring of the culture state. Conventional culture evaluation methods consist of end-point laborious techniques, not capable of real-time operation and not suitable for three-dimensional cultures. Electrical Impedance Tomography (EIT) is a non-invasive imaging technique with high potential to be used in cell culture monitoring due to its biocompatibility, non-invasiveness, high temporal resolution, compact hardware, automatic operation, and high throughput. This review approaches the different hardware strategies for cell culture EIT that are presented in the literature, discussing the main components of the measurement system: excitation circuit, voltage/current sensing, switching stage, signal specifications, electrode configurations, measurement protocols, and calibration strategies. The different approaches are qualitatively discussed and compared, and design guidelines are proposed.
人细胞培养物是疾病的生物模型、药物开发和组织工程的强大实验室工具。然而，生物实验的成功往往取决于对培养状态的实时监测。传统的培养物评估方法包括终点费力的技术，不能实时操作，不适合三维培养物。电阻抗断层成像（EIT）是一种非侵入性成像技术，由于其生物相容性、非侵入性、高时间分辨率、紧凑的硬件、自动操作和高通量，具有在细胞培养监测中应用的巨大潜力。本综述探讨了文献中提出的用于细胞培养 EIT 的不同硬件策略，讨论了测量系统的主要组成部分：激励电路、电压/电流感测、开关级、信号规格、电极配置、测量协议和校准策略。对不同的方法进行了定性讨论和比较，并提出了设计准则。
