Ou Yangteng, Cao Shixiang, Zhang Jing, Dong Weiliang, Yang Zhugen, Yu Ziyi
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemical Engineering, Nanjing Tech University, 30 Puzhu South Road, Nanjing, 211816, PR China.
Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Trends Analyt Chem. 2021 Oct;143:116333. doi: 10.1016/j.trac.2021.116333. Epub 2021 May 18.
Infectious diseases caused by pathogenic microbes have posed a major health issue for the public, such as the ongoing COVID-19 global pandemic. In recent years, wastewater-based epidemiology (WBE) is emerging as an effective and unbiased method for monitoring public health. Despite its increasing importance, the advancement of WBE requires more competent and streamlined analytical platforms. Herein we discuss the interactions between WBE and droplet microfluidics, focusing on the analysis of pathogens in droplets, which is hard to be tackled by traditional analytical tools. We highlight research works from three aspects, namely, quantitation of pathogen biomarkers in droplets, single-cell analysis in droplets, and living cell biosensors in droplets, as well as providing future perspectives on the synergy between WBE and droplet microfluidics.
由致病微生物引起的传染病给公众带来了重大健康问题，例如当前的新冠疫情全球大流行。近年来，基于废水的流行病学（WBE）正成为一种监测公众健康的有效且无偏倚的方法。尽管其重要性日益凸显，但WBE的发展需要更高效且精简的分析平台。在此，我们讨论WBE与微滴微流控之间的相互作用，重点关注微滴中病原体的分析，这是传统分析工具难以解决的问题。我们从三个方面突出研究工作，即微滴中病原体生物标志物的定量、微滴中的单细胞分析以及微滴中的活细胞生物传感器，并对WBE与微滴微流控之间的协同作用提供未来展望。
