球形节杆菌中铜结合型胺氧化酶同二聚体的氮氧化物自旋标记物的双电子电子共振（DEER）和弛豫诱导双量子相干（RIDME）测量
DEER and RIDME Measurements of the Nitroxide-Spin Labelled Copper-Bound Amine Oxidase Homodimer from Arthrobacter Globiformis.
作者信息
Russell Hannah, Stewart Rachel, Prior Christopher, Oganesyan Vasily S, Gaule Thembaninkosi G, Lovett Janet E
机构信息
SUPA School of Physics and Astronomy and BSRC, University of St Andrews, St Andrews, KY16 9SS UK.
School of Chemistry, University of East Anglia, Norwich, NR4 7TJ UK.
出版信息
Appl Magn Reson. 2021;52(8):995-1015. doi: 10.1007/s00723-021-01321-6. Epub 2021 Mar 29.
UNLABELLED
In the study of biological structures, pulse dipolar spectroscopy (PDS) is used to elucidate spin-spin distances at nanometre-scale by measuring dipole-dipole interactions between paramagnetic centres. The PDS methods of Double Electron Electron Resonance (DEER) and Relaxation Induced Dipolar Modulation Enhancement (RIDME) are employed, and their results compared, for the measurement of the dipolar coupling between nitroxide spin labels and copper-II (Cu(II)) paramagnetic centres within the copper amine oxidase from (AGAO). The distance distribution results obtained indicate that two distinct distances can be measured, with the longer of these at c.a. 5 nm. Conditions for optimising the RIDME experiment such that it may outperform DEER for these long distances are discussed. Modelling methods are used to show that the distances obtained after data analysis are consistent with the structure of AGAO.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s00723-021-01321-6.
未标注
在生物结构研究中，脉冲偶极光谱法（PDS）用于通过测量顺磁中心之间的偶极 - 偶极相互作用来阐明纳米尺度的自旋 - 自旋距离。采用了双电子电子共振（DEER）和弛豫诱导偶极调制增强（RIDME）的PDS方法，并比较了它们的结果，用于测量来自嗜热栖热放线菌的铜胺氧化酶（AGAO）中氮氧自旋标记与铜 - II（Cu(II)）顺磁中心之间的偶极耦合。获得的距离分布结果表明可以测量到两个不同的距离，其中较长的约为5纳米。讨论了优化RIDME实验使其在这些长距离上优于DEER的条件。使用建模方法表明数据分析后获得的距离与AGAO的结构一致。
补充信息
在线版本包含可在10.1007/s00723 - 021 - 01321 - 6获取的补充材料。
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