Russell Hannah, Cura Robyn, Lovett Janet E
SUPA School of Physics and Astronomy and BSRC, University of St Andrews, St Andrews, United Kingdom.
Front Mol Biosci. 2022 Jun 1;9:915167. doi: 10.3389/fmolb.2022.915167. eCollection 2022.
Pulsed dipolar electron paramagnetic resonance (PDEPR) spectroscopy experiments measure the dipolar coupling, and therefore nanometer-scale distances and distance distributions, between paramagnetic centers. Of the family of PDEPR experiments, the most commonly used pulsed sequence is four-pulse double electron resonance (DEER, also known as PELDOR). There are several ways to analyze DEER data to extract distance distributions, and this may appear overwhelming at first. This work compares and reviews six of the packages, and a brief getting started guide for each is provided.
脉冲偶极电子顺磁共振（PDEPR）光谱实验测量顺磁中心之间的偶极耦合，从而测量纳米尺度的距离和距离分布。在PDEPR实验家族中，最常用的脉冲序列是四脉冲双电子共振（DEER，也称为PELDOR）。有几种分析DEER数据以提取距离分布的方法，一开始这可能会让人应接不暇。这项工作比较并综述了其中六个软件包，并为每个软件包提供了简要的入门指南。
