利用电子顺磁共振波谱学研究转录机制。
The use of EPR spectroscopy to study transcription mechanisms.
作者信息
Hofmann L, Mandato A, Saxena S, Ruthstein S
机构信息
Department of Chemistry and the Institute of Nanotechnology and Advanced Materials, Bar Ilan University, Ramat-Gan, Israel.
Department of Chemistry, University of Pittsburgh, Pittsburgh, PA USA.
出版信息
Biophys Rev. 2022 Oct 25;14(5):1141-1159. doi: 10.1007/s12551-022-01004-x. eCollection 2022 Oct.
Electron paramagnetic resonance (EPR) spectroscopy has become a promising structural biology tool to resolve complex and dynamic biological mechanisms and . Here, we focus on the advantages of continuous wave (CW) and pulsed EPR distance measurements to resolve transcription processes and protein-DNA interaction. The wide range of spin-labeling approaches that can be used to follow structural changes in both protein and DNA render EPR a powerful method to study protein-DNA interactions and structure-function relationships in other macromolecular complexes. EPR-derived data goes well beyond static structural information and thus serves as the method of choice if dynamic insight is needed. Herein, we describe the conceptual details of the theory and the methodology and illustrate the use of EPR to study the protein-DNA interaction of the copper-sensitive transcription factor, CueR.
电子顺磁共振（EPR）光谱已成为一种很有前景的结构生物学工具，用于解析复杂且动态的生物学机制。在此，我们聚焦于连续波（CW）和脉冲EPR距离测量在解析转录过程和蛋白质 - DNA相互作用方面的优势。可用于追踪蛋白质和DNA结构变化的多种自旋标记方法，使EPR成为研究蛋白质 - DNA相互作用以及其他大分子复合物中结构 - 功能关系的有力方法。EPR衍生的数据远远超出静态结构信息，因此在需要动态洞察时是首选方法。在此，我们描述该理论和方法的概念细节，并举例说明EPR在研究铜敏感转录因子CueR的蛋白质 - DNA相互作用中的应用。
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