Sherman D R, Lloyd R S, Chytil F
Proc Natl Acad Sci U S A. 1987 May;84(10):3209-13. doi: 10.1073/pnas.84.10.3209.
Cellular retinol-binding protein (CRBP) may be an important mediator of vitamin A action. We report here the identification of a cDNA clone corresponding to the rat CRBP gene. The cDNA is 695 nucleotides long, with an open reading frame corresponding to a protein of 134 amino acids. The deduced amino acid sequence is identical with that of rat CRBP. The nucleotide sequence shows 90.5% similarity with the human CRBP cDNA sequence. Genomic DNA analysis indicates that CRBP is present in one, or at most two, copies within the rat genome. Analysis of mRNA reveals a single species in every tissue tested and suggests that the isolated cDNA is full-length. Finally, when retinol-deficient rats are fed retinyl acetate for 4 hr, about 4-fold accumulation of CRBP-specific mRNA is observed in the lungs. This rapid effect suggests that the micronutrient retinol may directly influence the expression of its specific intracellular binding protein.
细胞视黄醇结合蛋白（CRBP）可能是维生素A作用的重要介质。我们在此报告了与大鼠CRBP基因对应的cDNA克隆的鉴定。该cDNA长695个核苷酸，具有对应于134个氨基酸蛋白质的开放阅读框。推导的氨基酸序列与大鼠CRBP的序列相同。核苷酸序列与人CRBP cDNA序列显示出90.5%的相似性。基因组DNA分析表明，CRBP在大鼠基因组中以一个或最多两个拷贝存在。mRNA分析显示在每个测试组织中只有一种，这表明分离的cDNA是全长的。最后，当给维生素A缺乏的大鼠喂食醋酸视黄酯4小时后，在肺中观察到CRBP特异性mRNA积累约4倍。这种快速效应表明微量营养素视黄醇可能直接影响其特异性细胞内结合蛋白的表达。
