Nonino Carla Barbosa, Noronha Natália Yumi, de Araújo Ferreira-Julio Maysa, Moriguchi Watanabe Lígia, Cassia Karen Francislaine, Ferreira Nicoletti Carolina, Rossi Welendorf Caroline, Salgado Junior Wilson, Rossi Silva Souza Dorotéia, Augusta de Souza Pinhel Marcela
Department of Health Science, Ribeirao Preto Medical School, University of São Paulo, Ribeirao Preto, Brazil.
Department of Molecular Biology, São José do Rio Preto Medical School, São José do Rio Preto, Brazil.
Front Nutr. 2021 Oct 13;8:628759. doi: 10.3389/fnut.2021.628759. eCollection 2021.
Matrix metalloproteinases (MMP) and their endogenous inhibitor, the tissue inhibitor of metalloproteinases (TIMP), are expressed in many different cell types and play an important role in physiologic and pathological degradation of extracellular matrix (ECM). Starting from these observations and considering the activation state of peripheral blood mononuclear cells (PBMCs) in obesity, we investigated the gene expression of metalloproteinases before and after Roux-en-Y gastric bypass (RYBG). The study was performed in the Ribeirão Preto Medical School University Hospital. Seventy-three women were divided into a study group (SG), composed of 53 individuals with severe obesity before and after 6 months of RYGB, and a control group (CG), composed of 20 normal-weight individuals. Anthropometric and body composition data were collected, and peripheral blood for ribonucleic acid (RNA) extraction. The biological samples were submitted to a quantitative real-time polymerase chain reaction to evaluate the expression of and genes. Alterations in weight loss, body mass index (BMI), and fat mass (FM) were observed after 6 months of RYGB ( < 0.05). A reduction of gene expression of was observed after 6 months of RYGB, contributing positively to the weight loss ( = 0.33 = 0.04). The enrichment analyses highlighted the interaction between and genes and the molecular pathways involving the ECM remodeling in the obesity condition. RYGB contributes significantly to weight loss, improved BMI, reduced FM, and reduced expression in PBMCs, which might contribute to the ECM remodeling in the obesity and could be useful as a circulating biomarker.
基质金属蛋白酶（MMP）及其内源性抑制剂金属蛋白酶组织抑制剂（TIMP）在许多不同细胞类型中表达，并在细胞外基质（ECM）的生理和病理降解中发挥重要作用。基于这些观察结果并考虑到肥胖症中外周血单核细胞（PBMC）的激活状态，我们研究了Roux-en-Y胃旁路术（RYBG）前后金属蛋白酶的基因表达。该研究在里贝朗普雷图医学院大学医院进行。73名女性被分为研究组（SG），由53名严重肥胖患者在RYGB手术前和术后6个月组成，以及对照组（CG），由20名体重正常的个体组成。收集人体测量和身体成分数据，并采集外周血用于核糖核酸（RNA）提取。将生物样本进行定量实时聚合酶链反应，以评估 和 基因的表达。RYGB术后6个月观察到体重减轻、体重指数（BMI）和脂肪量（FM）的变化（ < 0.05）。RYGB术后6个月观察到 基因表达降低，对体重减轻有积极作用（ = 0.33， = 0.04）。富集分析突出了 和 基因之间的相互作用以及肥胖状态下涉及ECM重塑的分子途径。RYGB对体重减轻、改善BMI、降低FM以及降低PBMCs中 的表达有显著贡献，这可能有助于肥胖症中的ECM重塑，并且可作为一种循环生物标志物。
