Dipartimento Biomedico di Medicina Interna e Specialistica, Università di Palermo, Palermo, Italy.
Dipartimento di Scienze Psicologiche, Pedagogiche, dell'Esercizio Fisico e della Formazione, Università di Palermo, Palermo, Italy.
Horm Metab Res. 2019 Jun;51(6):389-395. doi: 10.1055/a-0887-2770. Epub 2019 May 10.
The association between obesity and cardiovascular diseases has a multifactorial pathogenesis, including the synthesis of inflammatory molecules, the increase in oxidative stress and the dysregulation of the matrix metalloprotease (MMP) concentration and activity. In a group of adults with obesity, divided in 2 subgroups according to the body mass index (BMI), we examined lipid peroxidation, expressed as thiobarbituric acid-reactive substances (TBARS), protein oxidation, expressed as protein carbonyl groups (PCs), plasma gelatinases (MMP-2 and MMP-9), and their tissue inhibitors (TIMP-1 and TIMP-2). In the whole group, as well as in the 2 subgroups (with BMI 30-35 or BMI>35) of obese subjects, we observed an increase in TBARS, PCs, MMP-2, and MMP-9, and also TIMP-1 and TIMP-2 in comparison with the control group. A positive correlation between TBARS and PCs emerged in obese subjects and persisted after dividing obese subjects according to BMI. The correlation between MMP-2 and TIMP-2 was not statistically significant, while a significant correlation was present between MMP-9 and TIMP-1. The correlations between the markers of oxidative stress (TBARS and PCs) and those of the MMP/TIMP profile indicated a more marked influence of protein oxidation on MMPs and TIMPs in comparison with TBARS. The innovative aspect of our study was the simultaneous evaluation of oxidative stress markers and MMP/TIMP profile in adult obese subjects. We observed significant alterations and correlations that may negatively influence the clinical course of the disease.
肥胖与心血管疾病之间的关联具有多因素的发病机制，包括炎症分子的合成、氧化应激的增加以及基质金属蛋白酶 (MMP) 浓度和活性的失调。在一组根据体重指数 (BMI) 分为 2 个子组的肥胖成年人中，我们检查了脂质过氧化，用硫代巴比妥酸反应物质 (TBARS) 表示，蛋白质氧化，用蛋白质羰基基团 (PCs) 表示，血浆明胶酶 (MMP-2 和 MMP-9) 及其组织抑制剂 (TIMP-1 和 TIMP-2)。在整个组以及肥胖受试者的 2 个子组 (BMI 为 30-35 或 BMI>35) 中，与对照组相比，我们观察到 TBARS、PCs、MMP-2 和 MMP-9 增加，以及 TIMP-1 和 TIMP-2 增加。在肥胖受试者中，TBARS 和 PCs 之间存在正相关，并且在根据 BMI 对肥胖受试者进行分组后仍然存在。MMP-2 和 TIMP-2 之间的相关性没有统计学意义，而 MMP-9 和 TIMP-1 之间存在显著相关性。氧化应激标志物 (TBARS 和 PCs) 与 MMP/TIMP 谱之间的相关性表明，与 TBARS 相比，蛋白质氧化对 MMPs 和 TIMPs 的影响更为显著。我们研究的创新之处在于同时评估成年肥胖受试者的氧化应激标志物和 MMP/TIMP 谱。我们观察到了可能对疾病的临床过程产生负面影响的显著变化和相关性。
