Department of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, China.
National Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory of Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, China.
J Immunol Res. 2021 Oct 20;2021:8124593. doi: 10.1155/2021/8124593. eCollection 2021.
Periodontitis is an inflammatory disease of microbial etiology caused primarily by dysbiosis of the oral microbiota. Our aim was to compare variations in the composition of the oral microbiomes of youths with severe periodontitis according to gender.
Subgingival plaque samples collected from 17 patients with severe periodontitis (11 males and 6 females) were split for 16S rRNA gene sequencing. The composition, -diversity, and -diversity of the patients' oral microbiomes were compared between the males and the females. Linear discriminant analysis effect size (LEfSe) was used to analyze the specific taxa enriched in the two groups. Functional profiles (KEGG pathways) were obtained using PICRUSt based on 16S rRNA gene sequencing data.
The Chao1 index and phylogenetic diversity whole tree were significantly higher in males than in females. The Simpson and Shannon indices were not significantly different between the two groups. -Diversity suggested that the samples were reasonably divided into groups. The Kruskal-Wallis test based on the relative abundance of species, combined with the LEfSe analysis showed that the dominant bacteria in males were and , whereas the dominant bacteria in women were Fusobacteriales and . KEGG analysis predicted that the variation in the oral microbiome may be related to the immune system in women, whereas immune system diseases were the dominant pathway in men.
We found sex-specific differences in the oral microbiome in a sample of youths with severe periodontitis. The differences may be related to changes in immune homeostasis and lead to a better understanding of periodontitis.
牙周炎是一种由口腔微生物群落失调引起的微生物病因炎症性疾病。我们的目的是比较性别不同的重度牙周炎青少年口腔微生物组组成的变化。
从 17 名重度牙周炎患者（男性 11 名，女性 6 名）的龈下菌斑样本中采集样本，用于 16S rRNA 基因测序。比较了男性和女性患者口腔微生物组的组成、α多样性和β多样性。使用线性判别分析效应量（LEfSe）分析两组中富集的特定分类群。基于 16S rRNA 基因测序数据，使用 PICRUSt 获得功能谱（KEGG 途径）。
男性的 Chao1 指数和全树系统发育多样性明显高于女性。辛普森和香农指数在两组之间无显著差异。β多样性表明样本被合理地分为组。基于物种相对丰度的 Kruskal-Wallis 检验，结合 LEfSe 分析表明，男性的优势菌为 和 ，而女性的优势菌为 Fusobacteriales 和 。KEGG 分析预测，口腔微生物组的变化可能与女性的免疫系统有关，而免疫系统疾病是男性的主要途径。
我们在重度牙周炎青少年样本中发现了口腔微生物组的性别特异性差异。这些差异可能与免疫稳态的变化有关，并有助于更好地理解牙周炎。
